Fibroblast growth factor downregulates expression of a basic helix- loop-helix-type transcription factor, scleraxis, in a chondrocyte-like cell line, TC6

Toshiyuki Kawa-uchi, Akira Nifuji, Nobuko Mataga, Eric N. Olson, Jacky Bonaventure, Ken Ichi Shinomiya, Ying Liu, Masaki Noda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Scleraxis is a basic helix-loop-helix-type transcription factor that is expressed in sclerotome. Fibroblast growth factor (FGF) is one of the cytokines produced by the cells in skeletal tissues and is a potent modulator of skeletogenesis. The aim of this study was to examine the effects of FGF on the expression of scleraxis in chondrocyte-like cells, TC6. In these cells, scleraxis mRNA was constitutively expressed as a 1.2kb message at a high level in contrast to its low levels of expression in fibroblast-like cells or osteoblast-like cells. Upon treatment with FGF, scleraxis mRNA level was decreased within 12 h. This effect was at its nadir at 24 h and the scleraxis mRNA level returned to its base line level by 48 h. The FGF effect was maximal at 1 ng/ml. FGF effects on scleraxis were blocked by actinomycin D but not by cycloheximide, suggesting the involvement of transcriptional events that do not require new protein synthesis. The FGF effects on scleraxis were blocked by genistein, suggesting the involvement of tyrosine kinase in the post-receptor signaling. TGFβ treatment of TC6 cells enhanced scleraxis mRNA expression; however, combination of the saturation doses of FGF and TGFβ resulted in suppression of scleraxis mRNA level. BMP2 also suppressed scleraxis mRNA expression in TC6 cells and no further suppression was observed in combination with FGF. These results indicate that scleraxis is expressed in chondrocyte-like TC6 cells and it is one of the targets of FGF action in these cells.

Original languageEnglish (US)
Pages (from-to)468-477
Number of pages10
JournalJournal of Cellular Biochemistry
Volume70
Issue number4
DOIs
StatePublished - Sep 15 1998

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Basic Helix-Loop-Helix Transcription Factors
Fibroblast Growth Factors
Chondrocytes
Down-Regulation
Cells
Cell Line
Messenger RNA
Genistein
Osteoblasts
Dactinomycin
Fibroblasts
Cycloheximide
Protein-Tyrosine Kinases
Modulators
Tissue
Cytokines

Keywords

  • BHLH
  • Chondrocyte
  • FGF
  • Scleraxis
  • Transcription factor

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Fibroblast growth factor downregulates expression of a basic helix- loop-helix-type transcription factor, scleraxis, in a chondrocyte-like cell line, TC6. / Kawa-uchi, Toshiyuki; Nifuji, Akira; Mataga, Nobuko; Olson, Eric N.; Bonaventure, Jacky; Shinomiya, Ken Ichi; Liu, Ying; Noda, Masaki.

In: Journal of Cellular Biochemistry, Vol. 70, No. 4, 15.09.1998, p. 468-477.

Research output: Contribution to journalArticle

Kawa-uchi, Toshiyuki ; Nifuji, Akira ; Mataga, Nobuko ; Olson, Eric N. ; Bonaventure, Jacky ; Shinomiya, Ken Ichi ; Liu, Ying ; Noda, Masaki. / Fibroblast growth factor downregulates expression of a basic helix- loop-helix-type transcription factor, scleraxis, in a chondrocyte-like cell line, TC6. In: Journal of Cellular Biochemistry. 1998 ; Vol. 70, No. 4. pp. 468-477.
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AU - Mataga, Nobuko

AU - Olson, Eric N.

AU - Bonaventure, Jacky

AU - Shinomiya, Ken Ichi

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AU - Noda, Masaki

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