Fibroblast mechanics in 3D collagen matrices

Sangmyung Rhee, Frederick Grinnell

Research output: Contribution to journalReview article

130 Scopus citations

Abstract

Connective tissues provide mechanical support and frameworks for the other tissues of the body. Type 1 collagen is the major protein component of ordinary connective tissue, and fibroblasts are the cell type primarily responsible for its biosynthesis and remodeling. Research on fibroblasts interacting with collagen matrices explores all four quadrants of cell mechanics: pro-migratory vs. pro-contractile growth factor environments on one axis; high tension vs. low tension cell-matrix interactions on the other. The dendritic fibroblast - probably equivalent to the resting tissue fibroblast - can be observed only in the low tension quadrant and generally has not been appreciated from research on cells incubated with planar culture surfaces. Fibroblasts in the low tension quadrant require microtubules for formation of dendritic extensions, whereas fibroblasts in the high tension quadrant require microtubules for polarization but not for spreading. Ruffling of dendritic extensions rather than their overall protrusion or retraction provides the mechanism for remodeling of floating collagen matrices, and floating matrix remodeling likely reflects a model of tissue mechanical homeostasis.

Original languageEnglish (US)
Pages (from-to)1299-1305
Number of pages7
JournalAdvanced Drug Delivery Reviews
Volume59
Issue number13
DOIs
StatePublished - Nov 10 2007

Keywords

  • Adhesion
  • Contraction
  • Extracellular matrix
  • Lysophosphatidic acid
  • Migration
  • Platelet-derived growth factor
  • Tensegrity
  • Tissue engineering
  • Wound repair

ASJC Scopus subject areas

  • Pharmaceutical Science

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