Fibroblast receptor for cell-substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin)

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Abstract

Studies were carried out on the interactions of uncharged latex beads (0.76 μm) with baby hamster kidney cells. Binding of beads to the cells occurred if the beads were coated by cold insoluble globulin (CIG) (plasma fibronectin) but not if the beads were coated by bovine albumin. Bovine albumin-coated beads did not bind to the cells even in the presence of excess CIG in the incubation medium. Binding of beads occurred randomly over the entire surfaces of cells in suspension. However, cell receptors for CIG beads were no longer detectable on the upper surface of cells spread on CIG-coated tissue culture dishes. Binding of CIG beads to cells occurred at all temperatures tested from 4° to 37°C but the rate was lowest at 4°C. At 37°C, binding was accompanied by endocytosis and the beads were found inside vesicles which appeared to be lysosomes. There was also release of radioactivity from radiolabeled CIG beads during incubation with the cells at 37°C. Binding of CIG beads to cells did not require divalent cations. Finally, the cell receptor for CIG beads was lost after cell trypsinization. The data are discussed in terms of current ideas about the basis for cell adhesion.

Original languageEnglish (US)
Pages (from-to)104-112
Number of pages9
JournalJournal of Cell Biology
Volume86
Issue number1
StatePublished - 1980

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Microspheres
Fibronectins
Cell Adhesion
Cricetinae
Fibroblasts
Kidney
Albumins
Divalent Cations
Endocytosis
Lysosomes
Radioactivity
Suspensions
Temperature

ASJC Scopus subject areas

  • Cell Biology

Cite this

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abstract = "Studies were carried out on the interactions of uncharged latex beads (0.76 μm) with baby hamster kidney cells. Binding of beads to the cells occurred if the beads were coated by cold insoluble globulin (CIG) (plasma fibronectin) but not if the beads were coated by bovine albumin. Bovine albumin-coated beads did not bind to the cells even in the presence of excess CIG in the incubation medium. Binding of beads occurred randomly over the entire surfaces of cells in suspension. However, cell receptors for CIG beads were no longer detectable on the upper surface of cells spread on CIG-coated tissue culture dishes. Binding of CIG beads to cells occurred at all temperatures tested from 4° to 37°C but the rate was lowest at 4°C. At 37°C, binding was accompanied by endocytosis and the beads were found inside vesicles which appeared to be lysosomes. There was also release of radioactivity from radiolabeled CIG beads during incubation with the cells at 37°C. Binding of CIG beads to cells did not require divalent cations. Finally, the cell receptor for CIG beads was lost after cell trypsinization. The data are discussed in terms of current ideas about the basis for cell adhesion.",
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N2 - Studies were carried out on the interactions of uncharged latex beads (0.76 μm) with baby hamster kidney cells. Binding of beads to the cells occurred if the beads were coated by cold insoluble globulin (CIG) (plasma fibronectin) but not if the beads were coated by bovine albumin. Bovine albumin-coated beads did not bind to the cells even in the presence of excess CIG in the incubation medium. Binding of beads occurred randomly over the entire surfaces of cells in suspension. However, cell receptors for CIG beads were no longer detectable on the upper surface of cells spread on CIG-coated tissue culture dishes. Binding of CIG beads to cells occurred at all temperatures tested from 4° to 37°C but the rate was lowest at 4°C. At 37°C, binding was accompanied by endocytosis and the beads were found inside vesicles which appeared to be lysosomes. There was also release of radioactivity from radiolabeled CIG beads during incubation with the cells at 37°C. Binding of CIG beads to cells did not require divalent cations. Finally, the cell receptor for CIG beads was lost after cell trypsinization. The data are discussed in terms of current ideas about the basis for cell adhesion.

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