Fibronectin and fibrinolysis are not required for fibrin gel contraction by human skin fibroblasts

T. L. Tuan, F. Grinnell

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Human skin fibroblasts contracted fibrin gels in a time- and cell-dependent manner. Under optimal conditions, gel contraction amounted to more than 50% in 2 hr. Fibronectin did not promote contraction, and fibrinolysis was not required for contraction, although gels contracted without serum or aprotinin were lysed. Before contraction, fibrin was present in loosely packed, randomly organized fibrils. After contraction, the fibrils were more densely packed and aligned in the plane of cell spreading. Cycloheximide treatment of fibroblasts inhibited gel contraction in serum-free medium but not in serum-containing medium. Fibronectin could not substitute for serum in overcoming the cycloheximide effect. Binding sites for fibrin were distributed randomly over the cells' surfaces based on electron microscopic observations. Often small groups of fibrils were localized in indentations at the cell surface. Finally, peptides containing the arg-gly-asp-ser sequence inhibited gel contraction.

Original languageEnglish (US)
Pages (from-to)577-583
Number of pages7
JournalJournal of Cellular Physiology
Volume140
Issue number3
StatePublished - 1989

Fingerprint

Fibrinolysis
Fibroblasts
Fibrin
Fibronectins
Skin
Gels
Cycloheximide
arginyl-glycyl-aspartyl-serine
Serum
Aprotinin
Serum-Free Culture Media
Binding Sites
Electrons
Indentation
Peptides

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Fibronectin and fibrinolysis are not required for fibrin gel contraction by human skin fibroblasts. / Tuan, T. L.; Grinnell, F.

In: Journal of Cellular Physiology, Vol. 140, No. 3, 1989, p. 577-583.

Research output: Contribution to journalArticle

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