Fibronectin and fibrinolysis are not required for fibrin gel contraction by human skin fibroblasts

T. L. Tuan, F. Grinnell

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Human skin fibroblasts contracted fibrin gels in a time‐ and cell‐dependent manner. Under optimal conditions, gel contraction amounted to more than 50% in 2 hr. Fibronectin did not promote contraction, and fibrinolysis was not required for contraction, although gels contracted without serum or aprotinin were lysed. Before contraction, fibrin was present in loosely packed, randomly organized fibrils. After contraction, the fibrils were more densely packed and aligned in the plane of cell spreading. Cycloheximide treatment of fibroblasts inhibited gel contraction in serum‐free medium but not in serum‐containing medium. Fibronectin could not substitute for serum in overcoming the cycloheximide effect. Binding sites for fibrin were distributed randomly over the cells' surfaces based on electron microscopic observations. Often small groups of fibrils were localized in indentations at the cell surface. Finally, peptides containing the arg‐gly‐asp‐ser sequence inhibited gel contraction.

Original languageEnglish (US)
Pages (from-to)577-583
Number of pages7
JournalJournal of cellular physiology
Volume140
Issue number3
DOIs
StatePublished - Sep 1989

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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