Field evaluation of a dual rapid immunodiagnostic test for HIV and syphilis infection in Peru

Claire C. Bristow, Segundo R. Leon, Emily Huang, Lourdes B. Ramos, Silver K. Vargas, Juan A. Flores, Kelika A. Konda, Carlos F. Caceres, Jeffrey D. Klausner

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background Integrated prevention for HIV and syphilis is warranted because both syphilis and HIV infections have evidence-based, scalable interventions using current health care mechanisms. The advent of dual rapid point-of-care tests, single devices that can detect multiple infections using the same specimen, provides the opportunity to integrate the screening of syphilis into HIV prevention programs, potentially increasing the numbers of people tested and allowing for same-day testing and treatment. The aim of our study was to evaluate the MedMira Multiplo Rapid TP/HIV Antibody Test (MedMira Inc, Halifax, Nova Scotia, Canada), a qualitative, rapid immunoassay that detects antibodies to Treponema pallidum and HIV. Methods The reference test for comparison to the T. pallidum component of the Multiplo TP/HIV Test was the T. pallidum particle agglutination assay. For the HIV component, the reference test included a fourth-generation enzyme immunoassay with a confirmatory Western blot test. Results The sensitivity and specificity for the HIV antibody component were 93.8% (95% confidence interval [CI], 69.8%-99.8%) and 100% (95% CI, 97.7%-100%), respectively. The T. pallidum component of the test had a sensitivity of 81.0% (95% CI, 68.1%-94.6%) and a specificity of 100% (95% CI, 97.6%-100%). Conclusions Our study showed excellent performance of the HIV antibody component of the test and very good performance for the T. pallidum antibody component of the MedMira Multiplo Rapid TP/HIV Antibody Test, which should be considered to improve screening coverage. Use of effective dual tests will create improved access to more comprehensive care by integrating the screening of syphilis into HIV prevention programs.

Original languageEnglish (US)
Pages (from-to)57-60
Number of pages4
JournalSexually Transmitted Diseases
Volume43
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Peru
Treponema pallidum
Syphilis
HIV Infections
HIV Antibodies
HIV
Confidence Intervals
Point-of-Care Systems
Nova Scotia
Antibodies
Agglutination
Immunoenzyme Techniques
Immunoassay
Canada
Western Blotting
Delivery of Health Care
Sensitivity and Specificity
Equipment and Supplies
Infection

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Bristow, C. C., Leon, S. R., Huang, E., Ramos, L. B., Vargas, S. K., Flores, J. A., ... Klausner, J. D. (2016). Field evaluation of a dual rapid immunodiagnostic test for HIV and syphilis infection in Peru. Sexually Transmitted Diseases, 43(1), 57-60. https://doi.org/10.1097/OLQ.0000000000000387

Field evaluation of a dual rapid immunodiagnostic test for HIV and syphilis infection in Peru. / Bristow, Claire C.; Leon, Segundo R.; Huang, Emily; Ramos, Lourdes B.; Vargas, Silver K.; Flores, Juan A.; Konda, Kelika A.; Caceres, Carlos F.; Klausner, Jeffrey D.

In: Sexually Transmitted Diseases, Vol. 43, No. 1, 01.01.2016, p. 57-60.

Research output: Contribution to journalArticle

Bristow, CC, Leon, SR, Huang, E, Ramos, LB, Vargas, SK, Flores, JA, Konda, KA, Caceres, CF & Klausner, JD 2016, 'Field evaluation of a dual rapid immunodiagnostic test for HIV and syphilis infection in Peru', Sexually Transmitted Diseases, vol. 43, no. 1, pp. 57-60. https://doi.org/10.1097/OLQ.0000000000000387
Bristow, Claire C. ; Leon, Segundo R. ; Huang, Emily ; Ramos, Lourdes B. ; Vargas, Silver K. ; Flores, Juan A. ; Konda, Kelika A. ; Caceres, Carlos F. ; Klausner, Jeffrey D. / Field evaluation of a dual rapid immunodiagnostic test for HIV and syphilis infection in Peru. In: Sexually Transmitted Diseases. 2016 ; Vol. 43, No. 1. pp. 57-60.
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abstract = "Background Integrated prevention for HIV and syphilis is warranted because both syphilis and HIV infections have evidence-based, scalable interventions using current health care mechanisms. The advent of dual rapid point-of-care tests, single devices that can detect multiple infections using the same specimen, provides the opportunity to integrate the screening of syphilis into HIV prevention programs, potentially increasing the numbers of people tested and allowing for same-day testing and treatment. The aim of our study was to evaluate the MedMira Multiplo Rapid TP/HIV Antibody Test (MedMira Inc, Halifax, Nova Scotia, Canada), a qualitative, rapid immunoassay that detects antibodies to Treponema pallidum and HIV. Methods The reference test for comparison to the T. pallidum component of the Multiplo TP/HIV Test was the T. pallidum particle agglutination assay. For the HIV component, the reference test included a fourth-generation enzyme immunoassay with a confirmatory Western blot test. Results The sensitivity and specificity for the HIV antibody component were 93.8{\%} (95{\%} confidence interval [CI], 69.8{\%}-99.8{\%}) and 100{\%} (95{\%} CI, 97.7{\%}-100{\%}), respectively. The T. pallidum component of the test had a sensitivity of 81.0{\%} (95{\%} CI, 68.1{\%}-94.6{\%}) and a specificity of 100{\%} (95{\%} CI, 97.6{\%}-100{\%}). Conclusions Our study showed excellent performance of the HIV antibody component of the test and very good performance for the T. pallidum antibody component of the MedMira Multiplo Rapid TP/HIV Antibody Test, which should be considered to improve screening coverage. Use of effective dual tests will create improved access to more comprehensive care by integrating the screening of syphilis into HIV prevention programs.",
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N2 - Background Integrated prevention for HIV and syphilis is warranted because both syphilis and HIV infections have evidence-based, scalable interventions using current health care mechanisms. The advent of dual rapid point-of-care tests, single devices that can detect multiple infections using the same specimen, provides the opportunity to integrate the screening of syphilis into HIV prevention programs, potentially increasing the numbers of people tested and allowing for same-day testing and treatment. The aim of our study was to evaluate the MedMira Multiplo Rapid TP/HIV Antibody Test (MedMira Inc, Halifax, Nova Scotia, Canada), a qualitative, rapid immunoassay that detects antibodies to Treponema pallidum and HIV. Methods The reference test for comparison to the T. pallidum component of the Multiplo TP/HIV Test was the T. pallidum particle agglutination assay. For the HIV component, the reference test included a fourth-generation enzyme immunoassay with a confirmatory Western blot test. Results The sensitivity and specificity for the HIV antibody component were 93.8% (95% confidence interval [CI], 69.8%-99.8%) and 100% (95% CI, 97.7%-100%), respectively. The T. pallidum component of the test had a sensitivity of 81.0% (95% CI, 68.1%-94.6%) and a specificity of 100% (95% CI, 97.6%-100%). Conclusions Our study showed excellent performance of the HIV antibody component of the test and very good performance for the T. pallidum antibody component of the MedMira Multiplo Rapid TP/HIV Antibody Test, which should be considered to improve screening coverage. Use of effective dual tests will create improved access to more comprehensive care by integrating the screening of syphilis into HIV prevention programs.

AB - Background Integrated prevention for HIV and syphilis is warranted because both syphilis and HIV infections have evidence-based, scalable interventions using current health care mechanisms. The advent of dual rapid point-of-care tests, single devices that can detect multiple infections using the same specimen, provides the opportunity to integrate the screening of syphilis into HIV prevention programs, potentially increasing the numbers of people tested and allowing for same-day testing and treatment. The aim of our study was to evaluate the MedMira Multiplo Rapid TP/HIV Antibody Test (MedMira Inc, Halifax, Nova Scotia, Canada), a qualitative, rapid immunoassay that detects antibodies to Treponema pallidum and HIV. Methods The reference test for comparison to the T. pallidum component of the Multiplo TP/HIV Test was the T. pallidum particle agglutination assay. For the HIV component, the reference test included a fourth-generation enzyme immunoassay with a confirmatory Western blot test. Results The sensitivity and specificity for the HIV antibody component were 93.8% (95% confidence interval [CI], 69.8%-99.8%) and 100% (95% CI, 97.7%-100%), respectively. The T. pallidum component of the test had a sensitivity of 81.0% (95% CI, 68.1%-94.6%) and a specificity of 100% (95% CI, 97.6%-100%). Conclusions Our study showed excellent performance of the HIV antibody component of the test and very good performance for the T. pallidum antibody component of the MedMira Multiplo Rapid TP/HIV Antibody Test, which should be considered to improve screening coverage. Use of effective dual tests will create improved access to more comprehensive care by integrating the screening of syphilis into HIV prevention programs.

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