Flow cytometric evaluation of early invasive cervical cancer

J. P. Connor, D. S. Miller, K. D. Bauer, T. M. Murad, A. W. Rademaker, J. R. Lurain

Research output: Contribution to journalArticle

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Abstract

Objective: To define the role of flow cytometry as a prognostic indicator in early cancers of the uterine cervix. Methods: Flow cytometry was used to determine ploidy, DNA index, and S-phase fraction on 141 samples from the tumors of 53 women with stage IB cancers of the cervix treated by radical hysterectomy and pelvic lymphadenectomy as primary therapy. Multiple samples of the same tumor were analyzable for 47 (89%) of the subjects. One-way analysis of variance for the multiple samples was used to compare the heterogeneity of flow cytometry data, both within each tumor and between patients. Flow cytometry results, as well as previously described clinical and pathologic prognostic factors, were correlated to recurrence and survival using Cox regression hazard ratios and Kaplan-Meier estimates. Results: We found DNA aneuploidy in 25 (47%) of the cancers, with a mean (± standard error) DNA index of 1.52 ± 0.07. The mean S-phase fraction was 7.6 ± 0.4% for diploid tumors and 9.2 ± 0.4% for aneuploid tumors. The cancers from 24 women (45%) were homogeneously diploid, 18 (34%) were consistently aneuploid, and five (9%) had mixed diploid/aneuploid samples. Analysis of variance of the multiple samples for each woman revealed a greater standard deviation (SD) between patients than within any individual tumor for both DNA index (ratio of between SD to within SD 2.1; P < .0001) and S-phase fraction (ratio 1.6; P < .0001). Of the previously described clinical and pathologic prognostic factors, only depth of invasion, expressed as either percent of cervical wall thickness or as thirds, was correlated with recurrence or survival. Neither the DNA index nor S-phase traction correlated significantly with recurrence or survival. Conclusions: These results suggest that alterations in the DNA content or proliferative activity of early invasive cancers of the uterine cervix do not reflect biologic behavior in terms of recurrence or survival, and that this behavior is not due to tumor heterogeneity.

Original languageEnglish (US)
Pages (from-to)367-371
Number of pages5
JournalObstetrics and Gynecology
Volume81
Issue number3
StatePublished - 1993

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Uterine Cervical Neoplasms
Aneuploidy
S Phase
Neoplasms
DNA
Flow Cytometry
Diploidy
Recurrence
Survival
Analysis of Variance
Ploidies
Kaplan-Meier Estimate
Traction
Lymph Node Excision
Hysterectomy

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Connor, J. P., Miller, D. S., Bauer, K. D., Murad, T. M., Rademaker, A. W., & Lurain, J. R. (1993). Flow cytometric evaluation of early invasive cervical cancer. Obstetrics and Gynecology, 81(3), 367-371.

Flow cytometric evaluation of early invasive cervical cancer. / Connor, J. P.; Miller, D. S.; Bauer, K. D.; Murad, T. M.; Rademaker, A. W.; Lurain, J. R.

In: Obstetrics and Gynecology, Vol. 81, No. 3, 1993, p. 367-371.

Research output: Contribution to journalArticle

Connor, JP, Miller, DS, Bauer, KD, Murad, TM, Rademaker, AW & Lurain, JR 1993, 'Flow cytometric evaluation of early invasive cervical cancer', Obstetrics and Gynecology, vol. 81, no. 3, pp. 367-371.
Connor JP, Miller DS, Bauer KD, Murad TM, Rademaker AW, Lurain JR. Flow cytometric evaluation of early invasive cervical cancer. Obstetrics and Gynecology. 1993;81(3):367-371.
Connor, J. P. ; Miller, D. S. ; Bauer, K. D. ; Murad, T. M. ; Rademaker, A. W. ; Lurain, J. R. / Flow cytometric evaluation of early invasive cervical cancer. In: Obstetrics and Gynecology. 1993 ; Vol. 81, No. 3. pp. 367-371.
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abstract = "Objective: To define the role of flow cytometry as a prognostic indicator in early cancers of the uterine cervix. Methods: Flow cytometry was used to determine ploidy, DNA index, and S-phase fraction on 141 samples from the tumors of 53 women with stage IB cancers of the cervix treated by radical hysterectomy and pelvic lymphadenectomy as primary therapy. Multiple samples of the same tumor were analyzable for 47 (89{\%}) of the subjects. One-way analysis of variance for the multiple samples was used to compare the heterogeneity of flow cytometry data, both within each tumor and between patients. Flow cytometry results, as well as previously described clinical and pathologic prognostic factors, were correlated to recurrence and survival using Cox regression hazard ratios and Kaplan-Meier estimates. Results: We found DNA aneuploidy in 25 (47{\%}) of the cancers, with a mean (± standard error) DNA index of 1.52 ± 0.07. The mean S-phase fraction was 7.6 ± 0.4{\%} for diploid tumors and 9.2 ± 0.4{\%} for aneuploid tumors. The cancers from 24 women (45{\%}) were homogeneously diploid, 18 (34{\%}) were consistently aneuploid, and five (9{\%}) had mixed diploid/aneuploid samples. Analysis of variance of the multiple samples for each woman revealed a greater standard deviation (SD) between patients than within any individual tumor for both DNA index (ratio of between SD to within SD 2.1; P < .0001) and S-phase fraction (ratio 1.6; P < .0001). Of the previously described clinical and pathologic prognostic factors, only depth of invasion, expressed as either percent of cervical wall thickness or as thirds, was correlated with recurrence or survival. Neither the DNA index nor S-phase traction correlated significantly with recurrence or survival. Conclusions: These results suggest that alterations in the DNA content or proliferative activity of early invasive cancers of the uterine cervix do not reflect biologic behavior in terms of recurrence or survival, and that this behavior is not due to tumor heterogeneity.",
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AU - Connor, J. P.

AU - Miller, D. S.

AU - Bauer, K. D.

AU - Murad, T. M.

AU - Rademaker, A. W.

AU - Lurain, J. R.

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N2 - Objective: To define the role of flow cytometry as a prognostic indicator in early cancers of the uterine cervix. Methods: Flow cytometry was used to determine ploidy, DNA index, and S-phase fraction on 141 samples from the tumors of 53 women with stage IB cancers of the cervix treated by radical hysterectomy and pelvic lymphadenectomy as primary therapy. Multiple samples of the same tumor were analyzable for 47 (89%) of the subjects. One-way analysis of variance for the multiple samples was used to compare the heterogeneity of flow cytometry data, both within each tumor and between patients. Flow cytometry results, as well as previously described clinical and pathologic prognostic factors, were correlated to recurrence and survival using Cox regression hazard ratios and Kaplan-Meier estimates. Results: We found DNA aneuploidy in 25 (47%) of the cancers, with a mean (± standard error) DNA index of 1.52 ± 0.07. The mean S-phase fraction was 7.6 ± 0.4% for diploid tumors and 9.2 ± 0.4% for aneuploid tumors. The cancers from 24 women (45%) were homogeneously diploid, 18 (34%) were consistently aneuploid, and five (9%) had mixed diploid/aneuploid samples. Analysis of variance of the multiple samples for each woman revealed a greater standard deviation (SD) between patients than within any individual tumor for both DNA index (ratio of between SD to within SD 2.1; P < .0001) and S-phase fraction (ratio 1.6; P < .0001). Of the previously described clinical and pathologic prognostic factors, only depth of invasion, expressed as either percent of cervical wall thickness or as thirds, was correlated with recurrence or survival. Neither the DNA index nor S-phase traction correlated significantly with recurrence or survival. Conclusions: These results suggest that alterations in the DNA content or proliferative activity of early invasive cancers of the uterine cervix do not reflect biologic behavior in terms of recurrence or survival, and that this behavior is not due to tumor heterogeneity.

AB - Objective: To define the role of flow cytometry as a prognostic indicator in early cancers of the uterine cervix. Methods: Flow cytometry was used to determine ploidy, DNA index, and S-phase fraction on 141 samples from the tumors of 53 women with stage IB cancers of the cervix treated by radical hysterectomy and pelvic lymphadenectomy as primary therapy. Multiple samples of the same tumor were analyzable for 47 (89%) of the subjects. One-way analysis of variance for the multiple samples was used to compare the heterogeneity of flow cytometry data, both within each tumor and between patients. Flow cytometry results, as well as previously described clinical and pathologic prognostic factors, were correlated to recurrence and survival using Cox regression hazard ratios and Kaplan-Meier estimates. Results: We found DNA aneuploidy in 25 (47%) of the cancers, with a mean (± standard error) DNA index of 1.52 ± 0.07. The mean S-phase fraction was 7.6 ± 0.4% for diploid tumors and 9.2 ± 0.4% for aneuploid tumors. The cancers from 24 women (45%) were homogeneously diploid, 18 (34%) were consistently aneuploid, and five (9%) had mixed diploid/aneuploid samples. Analysis of variance of the multiple samples for each woman revealed a greater standard deviation (SD) between patients than within any individual tumor for both DNA index (ratio of between SD to within SD 2.1; P < .0001) and S-phase fraction (ratio 1.6; P < .0001). Of the previously described clinical and pathologic prognostic factors, only depth of invasion, expressed as either percent of cervical wall thickness or as thirds, was correlated with recurrence or survival. Neither the DNA index nor S-phase traction correlated significantly with recurrence or survival. Conclusions: These results suggest that alterations in the DNA content or proliferative activity of early invasive cancers of the uterine cervix do not reflect biologic behavior in terms of recurrence or survival, and that this behavior is not due to tumor heterogeneity.

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