Flow cytometry for quantification of retrogradely labeled retinal ganglion cells by Fluoro-Gold

Junjie Yang, Gülgün Tezel, Rajkumar V. Patil, Martin B. Wax

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose. To count retrogradely labeled retinal ganglion cells by Fluoro-Gold. Methods. Retinal ganglion cells were retrogradely labeled using bilateral injections of Fluoro-Gold into the superior colliculus. One week after injections, retinas were dissociated and immunolabeled using specific antibody against Fluoro-Gold. The Fluoro-Gold labeled cells were then counted using flow cytometry. Results. Flow cytometry revealed that approximately 7% of the dissociated retinal cells were ganglion cells retrogradely labeled by Fluoro-Gold (Fig. 1B). Based on the total count of retinal cells per eye, the total number of retinal ganglion cells was estimated at approximately 131,250 ± 2,542 per rat eye. The coefficient of variation of counts was calculated as 1.98%. Conclusions. The use of flow cytometry facilitates simple, reproducible and rapid quantification of virtually all of the retinal ganglion cells labeled by Fluoro-Gold in a single rat eye.

Original languageEnglish (US)
Pages (from-to)981-985
Number of pages5
JournalCurrent Eye Research
Volume21
Issue number6
DOIs
StatePublished - Dec 2000

Fingerprint

Retinal Ganglion Cells
Flow Cytometry
Injections
Superior Colliculi
Retina
methanesulfonate salt 2-hydroxy-4,4'-diamidinostilbene
Cell Count
Antibodies

Keywords

  • Anti-Fluoro-Gold antibody
  • Flow cytometry
  • Fluoro-Gold
  • Retinal ganglion cells
  • Retrograde labeling

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Flow cytometry for quantification of retrogradely labeled retinal ganglion cells by Fluoro-Gold. / Yang, Junjie; Tezel, Gülgün; Patil, Rajkumar V.; Wax, Martin B.

In: Current Eye Research, Vol. 21, No. 6, 12.2000, p. 981-985.

Research output: Contribution to journalArticle

Yang, Junjie ; Tezel, Gülgün ; Patil, Rajkumar V. ; Wax, Martin B. / Flow cytometry for quantification of retrogradely labeled retinal ganglion cells by Fluoro-Gold. In: Current Eye Research. 2000 ; Vol. 21, No. 6. pp. 981-985.
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