Fluorescence associated with the type 3 copper center of laccase

R. Max Wynn, Hemanta K. Sarkar, Robert A. Holwerda, David B. Knaff

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Laccases isolated from the lacquer tree Rhus vernicifera and the fungus Polyporus versicolor show fluorescence emission near 420 nm and phosphorescence emission in the 440-465 nm region. The fluorescence and phosphorescence excitation spectra for both laccases show maxima in the 315-330 nm range, a spectral region corresponding to the absorbance maxima for the type 3 binuclear Cu centers of the two enzymes. Additional evidence is presented for the association of the newly discovered emissions with the type 3 Cu centers of the two laccases.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalFEBS Letters
Volume156
Issue number1
DOIs
StatePublished - May 30 1983

Keywords

  • Fluorescence
  • Laccase
  • Phosphorescence
  • Type 3 Cu

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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