Fluorescence correlation spectroscopy and quantitative cell biology

Mikhail K. Levin; John H. Carson

doi:10.1111/j.1432-0436.2004.07201002.x

Fluorescence correlation spectroscopy and quantitative cell biology

Mikhail K. Levin, John H. Carson

Clinical Sciences

Research output: Contribution to journal › Review article › peer-review

35 Scopus citations

Abstract

Fluorescence correlation spectroscopy (FCS) analyzes fluctuations in fluorescence within a small observation volume. Autocorrelation analysis of FCS fluctuation data can be used to measure concentrations, diffusion properties, and kinetic constants for individual fluorescent molecules. Photon count histogram analysis of fluorescence fluctuation data can be used to study oligomerization of individual fluorescent molecules. If the FCS observation volume is positioned inside a living cell, these parameters can be measured in vivo. FCS can provide the requisite quantitative data for analysis of molecular interaction networks underlying complex cell biological processes.

Original language	English (US)
Pages (from-to)	1-10
Number of pages	10
Journal	Differentiation
Volume	72
Issue number	1
DOIs	https://doi.org/10.1111/j.1432-0436.2004.07201002.x
State	Published - Feb 2004

Keywords

Avalanche photodiode
Fluorescence correlation spectroscopy
Green fluorescent protein
Photon count histogram

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology
Cancer Research

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10.1111/j.1432-0436.2004.07201002.x

Cite this

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title = "Fluorescence correlation spectroscopy and quantitative cell biology",

abstract = "Fluorescence correlation spectroscopy (FCS) analyzes fluctuations in fluorescence within a small observation volume. Autocorrelation analysis of FCS fluctuation data can be used to measure concentrations, diffusion properties, and kinetic constants for individual fluorescent molecules. Photon count histogram analysis of fluorescence fluctuation data can be used to study oligomerization of individual fluorescent molecules. If the FCS observation volume is positioned inside a living cell, these parameters can be measured in vivo. FCS can provide the requisite quantitative data for analysis of molecular interaction networks underlying complex cell biological processes.",

keywords = "Avalanche photodiode, Fluorescence correlation spectroscopy, Green fluorescent protein, Photon count histogram",

author = "Levin, {Mikhail K.} and Carson, {John H.}",

note = "Funding Information: Acknowledgments This work was supported by NIH grants NS15190 and RR13186 to J.H.C. We would also like to acknowledge Allison C. Paradise (UCHC), Boris Slepchenko (UCHC), Igor Novak (UCHC), and Klaus Wei≈hart (Zeiss) for helpful discussions.",

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AU - Carson, John H.

N1 - Funding Information: Acknowledgments This work was supported by NIH grants NS15190 and RR13186 to J.H.C. We would also like to acknowledge Allison C. Paradise (UCHC), Boris Slepchenko (UCHC), Igor Novak (UCHC), and Klaus Wei≈hart (Zeiss) for helpful discussions.

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N2 - Fluorescence correlation spectroscopy (FCS) analyzes fluctuations in fluorescence within a small observation volume. Autocorrelation analysis of FCS fluctuation data can be used to measure concentrations, diffusion properties, and kinetic constants for individual fluorescent molecules. Photon count histogram analysis of fluorescence fluctuation data can be used to study oligomerization of individual fluorescent molecules. If the FCS observation volume is positioned inside a living cell, these parameters can be measured in vivo. FCS can provide the requisite quantitative data for analysis of molecular interaction networks underlying complex cell biological processes.

AB - Fluorescence correlation spectroscopy (FCS) analyzes fluctuations in fluorescence within a small observation volume. Autocorrelation analysis of FCS fluctuation data can be used to measure concentrations, diffusion properties, and kinetic constants for individual fluorescent molecules. Photon count histogram analysis of fluorescence fluctuation data can be used to study oligomerization of individual fluorescent molecules. If the FCS observation volume is positioned inside a living cell, these parameters can be measured in vivo. FCS can provide the requisite quantitative data for analysis of molecular interaction networks underlying complex cell biological processes.

KW - Avalanche photodiode

KW - Fluorescence correlation spectroscopy

KW - Green fluorescent protein

KW - Photon count histogram

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Fluorescence correlation spectroscopy and quantitative cell biology

Abstract

Keywords

ASJC Scopus subject areas

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