Fluorescence correlation spectroscopy and quantitative cell biology

Mikhail K. Levin, John H. Carson

Research output: Contribution to journalReview article

31 Scopus citations

Abstract

Fluorescence correlation spectroscopy (FCS) analyzes fluctuations in fluorescence within a small observation volume. Autocorrelation analysis of FCS fluctuation data can be used to measure concentrations, diffusion properties, and kinetic constants for individual fluorescent molecules. Photon count histogram analysis of fluorescence fluctuation data can be used to study oligomerization of individual fluorescent molecules. If the FCS observation volume is positioned inside a living cell, these parameters can be measured in vivo. FCS can provide the requisite quantitative data for analysis of molecular interaction networks underlying complex cell biological processes.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalDifferentiation
Volume72
Issue number1
DOIs
StatePublished - Feb 2004

Keywords

  • Avalanche photodiode
  • Fluorescence correlation spectroscopy
  • Green fluorescent protein
  • Photon count histogram

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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