Fluorescence fluctuation spectroscopy approaches to the study of receptors in live cells

David M. Jameson, Nicholas G. James, Joseph P. Albanesi

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations

Abstract

Communication between cells and their environment, including other cells, is often mediated by cell surface receptors. Fluorescence methodologies are among the most important techniques used to study receptors and their interactions, and in the past decade, fluorescence fluctuation spectroscopy (FFS) approaches have been increasingly utilized. In this overview, we illustrate how diverse FFS approaches have been used to elucidate important aspects of receptor systems, including interactions of receptors with their ligands and receptor oligomerization and clustering. We also describe the most popular methods used to introduce fluorescent moieties into the biological systems. Finally, specific attention will be given to cell maintenance and transfection strategies especially as related to microscopy studies.

Original languageEnglish (US)
Title of host publicationFluorescence Fluctuation Spectroscopy (FFS), Part B
PublisherAcademic Press Inc.
Pages87-113
Number of pages27
ISBN (Print)9780124055391
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume519
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Fluorescence fluctuation spectroscopy
  • GPCR
  • autofluorescence
  • cell growth
  • fluorophores
  • receptors
  • transfection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Jameson, D. M., James, N. G., & Albanesi, J. P. (2013). Fluorescence fluctuation spectroscopy approaches to the study of receptors in live cells. In Fluorescence Fluctuation Spectroscopy (FFS), Part B (pp. 87-113). (Methods in Enzymology; Vol. 519). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-405539-1.00003-8