Fluorescence properties of tetrahydrofolate and related compounds

Kosaku Uyeda, Jesse C. Rabinowitz

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

A survey of fluorescence characteristics of tetrahydrofolate and related compounds has been carried out with an Aminco-Bowman Spectrophotofluorometer. Dihydrofolate and all the tetrahydrofolate derivatives examined, except 5,10-methenyltetrahydrofolate, show maximum excitation between 300 to 320 mμ and maximum fluorescence between 360 to 425 mμ. On the other hand, folate and the related compounds exhibit an excitation maximum at 360 to 380 mμ and a fluorescence maximum at 450 to 460 mμ. The fluorescence intensity of tetrahydrofolate increases markedly in acidic solutions. This property provides a simple and a highly sensitive method for a quantitative determination of tetrahydrofolate.

Original languageEnglish (US)
Pages (from-to)100-108
Number of pages9
JournalAnalytical biochemistry
Volume6
Issue number1
DOIs
StatePublished - Jul 1963

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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