Fluorescence studies of nucleotide interactions with bovine adrenal chromogranin A

Seung H. Yoo, Joseph P. Albanesi, David M. Jameson

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The binding of the fluorescent probe bis-ANS to chromogranin A, the major protein of adrenal chromaffin vesicles, caused a marked enhancement and blue shift in the fluorescence emission spectrum. The emission maximum shifted from 515 nm to 480 nm and the yield increased approx. 75-fold upon addition of 10 μM chromogranin A to 1 μM bis-ANS. Adenine nucleotides had clear effects on the bis-ANS fluorescence signal, while other nucleotides such as GTP, UTP and CTP had no discernible effect. Specifically, ATP caused a decrease in the fluorescence, whereas ADP and AMP caused a fluorescence increase. These results indicate adenine nucleotide binding to chromogranin A. Substitution of ATP with ε-ATP, an ATP derivative with a modification on the six-membered ring of the adenine base, failed to reduce the fluorescence intensity. Therefore, it was concluded that adenine bases play an important role in the chromogranin A-adenine nucleotide interaction.

Original languageEnglish (US)
Pages (from-to)66-70
Number of pages5
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1040
Issue number1
DOIs
StatePublished - Aug 1 1990

Keywords

  • Adenine nucleotide binding
  • Bis-ANS fluorescence
  • Chromogranin A

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Fluorescence studies of nucleotide interactions with bovine adrenal chromogranin A'. Together they form a unique fingerprint.

  • Cite this