Fluoride rapidly and transiently raises intracellular calcium in human osteoblasts

Joseph E. Zerwekh, Alan C. Morris, Paulette K. Padalino, Frank Gottschalk, Charles Y C Pak

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We examined the effect of fluoride (F) on intracellular ionic calcium [Ca2+]i in normal human osteoblasts maintained in culture. Cells were grown on glass coverslips to near-confluency and loaded with the Ca-sensitive dye, fura-2AM. Fluorescence changes were monitored in single cells using an inverted microscope coupled by fiberoptic to a microspectrofluoremeter. The addition of F (100 ng/niL) to the medium promoted a rapid and significant increase in free [Ca2+]i from a resting level of 245 ± 36 SE nM to a peak concentration of 440 ± 51 nM (p < 0.04). This increase in [Ca2+]i began at 10-20 s after addition of F and was maximal by 30 s. Intracellular [Ca2+], levels then returned to near resting values by 60-80 s after F addition. This response was evident with as little as 25 ng/ml of fluoride and was dose dependent up to 500 ng/ml. At concentrations greater than 500 ng/ml, there appeared to be an attenuation of the rise in [Ca2+]i. The observed rise in [Ca2+]i was dependent on extracellular calcium since lowering extracellular calcium concentration or incubation with calcium channel blockers abolished the response. This observation supports a role of increased [Ca2+]i as one of the initial events of fluoride on action osteoblasts.

Original languageEnglish (US)
JournalJournal of Bone and Mineral Research
Volume5
Issue numberSUPPL. 1
StatePublished - Mar 1990

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Osteoblasts
Fluorides
Calcium
Calcium Channel Blockers
Glass
Coloring Agents
Fluorescence

ASJC Scopus subject areas

  • Surgery

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Fluoride rapidly and transiently raises intracellular calcium in human osteoblasts. / Zerwekh, Joseph E.; Morris, Alan C.; Padalino, Paulette K.; Gottschalk, Frank; Pak, Charles Y C.

In: Journal of Bone and Mineral Research, Vol. 5, No. SUPPL. 1, 03.1990.

Research output: Contribution to journalArticle

Zerwekh, Joseph E. ; Morris, Alan C. ; Padalino, Paulette K. ; Gottschalk, Frank ; Pak, Charles Y C. / Fluoride rapidly and transiently raises intracellular calcium in human osteoblasts. In: Journal of Bone and Mineral Research. 1990 ; Vol. 5, No. SUPPL. 1.
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