Fluoride-stimulated [3H]thymidine uptake in a human osteoblastic osteosarcoma cell line is dependent on transforming growth factor β

Berenice Y. Reed, Joseph E. Zerwekh, Peter P. Antich, Charles Y C Pak

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Controversy exists regarding the effect of fluoride on human osteoblast proliferation. To learn more of the cellular action of fluoride, we chose the clonal osteoblast cell line HOS TE85 as a model system. In these phenotypically osteoblast-like cells, sodium fluoride stimulated [3H]thymidine incorporation in a dose-dependent manner over the concentration range 1 × 10-5-2 × 10-4 M. The fluoride-induced stimulation of [3H]thymidine uptake was dependent on cell density, being optimal at subconfluent cell numbers. Stimulation of [3H]thymidine uptake was inhibited by anti-transforming growth factor β but not by antibody to insulin-like growth factor I or β2-microglobulin. Transforming growth factor β was shown to be a biphasic stimulator of [3H]thymidine uptake in HOS TE85, with maximal stimulation occurring at 0.5 nM transforming growth factor β. In the presence of fluoride the cells were more sensitive to stimulation by this growth factor, with maximum effect occurring at 0.1 nM. Fluoride did not increase mRNA for transforming growth factor β following either 8 or 24 h of exposure. We conclude that fluoride activates osteoblast proliferation by modulating the cellular sensitivity to transforming growth factor β, a known stimulator of bone growth.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalJournal of Bone and Mineral Research
Volume8
Issue number1
StatePublished - Jan 1993

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Transforming Growth Factors
Osteosarcoma
Fluorides
Thymidine
Osteoblasts
Cell Line
Cell Count
Sodium Fluoride
Bone Development
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Messenger RNA
Antibodies

ASJC Scopus subject areas

  • Surgery

Cite this

Fluoride-stimulated [3H]thymidine uptake in a human osteoblastic osteosarcoma cell line is dependent on transforming growth factor β. / Reed, Berenice Y.; Zerwekh, Joseph E.; Antich, Peter P.; Pak, Charles Y C.

In: Journal of Bone and Mineral Research, Vol. 8, No. 1, 01.1993, p. 19-25.

Research output: Contribution to journalArticle

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