Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster

Reed J. Kelso, Michael Buszczak, Ana T. Quiñones, Claudia Castiblanco, Stacy Mazzalupo, Lynn Cooley

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Flytrap is a web-enabled relational database of transposable element insertions in Drosophila melanogaster. A green fluorescent protein (GFP) artificial exon carried by a transposable P-element is mobilized and inserted into a host gene intron creating a GFP fusion protein. The sequence of the tagged gene is determined by sequencing inverse-PCR products derived from genomic DNA. Flytrap contains two principle data types: micrographs of protein localization and a cellular component ontology, based on rules derived from the Gene Ontology consortium (http://www.geneontology.org), describing protein localization. Flytrap also has links to gene information contained in Flybase (http://flybase.bio.indiana.edu). The system is designed to accept submissions of micrographs and descriptions from any type of tissue (e.g. wing imaginal disk, ovary) and at any stage of development. Insertion lines can be searched using a number of queries, including Berkeley Drosophila Genome Project (BDGP) numbers and protein localization. In addition, Flytrap provides online order forms linked to each insertion line so that users may request any line generated from this project. Flytrap may be accessed from the homepage at http://flytrap.med.yale.edu.

Original languageEnglish (US)
JournalNucleic Acids Research
Volume32
Issue numberDATABASE ISS.
StatePublished - Jan 1 2004

Fingerprint

Sarraceniaceae
Green Fluorescent Proteins
Drosophila melanogaster
Databases
Proteins
DNA Transposable Elements
Genes
Imaginal Discs
Gene Ontology
Introns
Drosophila
Ovary
Exons
Genome
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Kelso, R. J., Buszczak, M., Quiñones, A. T., Castiblanco, C., Mazzalupo, S., & Cooley, L. (2004). Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster. Nucleic Acids Research, 32(DATABASE ISS.).

Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster. / Kelso, Reed J.; Buszczak, Michael; Quiñones, Ana T.; Castiblanco, Claudia; Mazzalupo, Stacy; Cooley, Lynn.

In: Nucleic Acids Research, Vol. 32, No. DATABASE ISS., 01.01.2004.

Research output: Contribution to journalArticle

Kelso, RJ, Buszczak, M, Quiñones, AT, Castiblanco, C, Mazzalupo, S & Cooley, L 2004, 'Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster', Nucleic Acids Research, vol. 32, no. DATABASE ISS..
Kelso RJ, Buszczak M, Quiñones AT, Castiblanco C, Mazzalupo S, Cooley L. Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster. Nucleic Acids Research. 2004 Jan 1;32(DATABASE ISS.).
Kelso, Reed J. ; Buszczak, Michael ; Quiñones, Ana T. ; Castiblanco, Claudia ; Mazzalupo, Stacy ; Cooley, Lynn. / Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster. In: Nucleic Acids Research. 2004 ; Vol. 32, No. DATABASE ISS.
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