Folding intermediates of snare complex assembly

K. M. Fiebig, L. M. Rice, E. Pollock, A. T. Brunger

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

SNARE (soluble NSF attachment protein receptor) proteins assemble into a stable complex essential for vesicle-membrane fusion. To further understand SNARE function we have used solution nuclear magnetic resonance (NMR) spectroscopy to characterize three assembly states of a yeast SNARE complex: first, the 'closed' conformation of Sso1; second, the binary complex of Sso1 and Sec9; and third, the ternary complex of Sso1, Sec9 and Snc1. Sec9 and Snc1 are unstructured in isolation. Sso1 likely consists of a four helix bundle formed by part of the C-terminal H(core) domain and the N-terminal H(A)H(B)H(C) domain, and this bundle is flanked on both sides by large flexible regions. Sso1 switches to an 'open' state when its H(core) domain binds Sec9. Conformational switching of the H(core) domain, via H(A)H(B)H(C), may provide a key regulatory mechanism in SNARE assembly. Formation of binary and ternary complexes induces additional α-helical structure in previously unstructured regions. Our data suggest a directed assembly process beginning distal to the membrane surfaces and proceeding toward them, bringing membranes into close proximity and possibly leading to membrane fusion.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalNature Structural Biology
Volume6
Issue number2
DOIs
StatePublished - 1999

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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
SNARE Proteins
Membranes
Membrane Fusion
Fusion reactions
Yeast
Nuclear magnetic resonance spectroscopy
Conformations
Magnetic Resonance Spectroscopy
Yeasts
Switches
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Folding intermediates of snare complex assembly. / Fiebig, K. M.; Rice, L. M.; Pollock, E.; Brunger, A. T.

In: Nature Structural Biology, Vol. 6, No. 2, 1999, p. 117-123.

Research output: Contribution to journalArticle

Fiebig, KM, Rice, LM, Pollock, E & Brunger, AT 1999, 'Folding intermediates of snare complex assembly', Nature Structural Biology, vol. 6, no. 2, pp. 117-123. https://doi.org/10.1038/5803
Fiebig, K. M. ; Rice, L. M. ; Pollock, E. ; Brunger, A. T. / Folding intermediates of snare complex assembly. In: Nature Structural Biology. 1999 ; Vol. 6, No. 2. pp. 117-123.
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