Follicle-stimulating hormone receptor is expressed in human ovarian surface epithelium and fallopian tube

Wenxin Zheng, Margret S. Magid, Elmer E. Kramer, Yao Tseng Chen

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The cellular expression of pituitary gonadotropin receptors in gonadal tissues is poorly defined because of the lack of suitable reagents. In this study, we developed in situ hybridization and reverse transcription polymerase chain reaction techniques for the evaluation of follicle- stimulating hormone receptor (FSHR) expression in the ovary and fallopian tube. Using a single-strand RNA probe, we demonstrated that FSHR mRNA expression is strongest in Graafian follicles. Within these developing follicles, granulosa cells showed the greatest expression, although both theca interna and theca externa were also positive, interne greater than externa. Granulosa cells in both primary and primordial follicles were positive, with primordial follicles showing only weak focal positivity. Ovarian surface epithelium and fallopian tube epithelium, not previously recognized to express FSHR, were both strongly positive. The FSHR expression in the ovary and fallopian tube was confirmed by reverse transcription polymerase chain reaction. Our results indicated that the FSHR is expressed in a cell-specific fashion at different stages of follicular development and is also expressed in ovarian surface and fallopian tube epithelia. The presence of FSHR in ovarian surface epithelium and of gonadotropin-binding sites in ovarian neoplasms provide additional evidence supporting the derivation of epithelial ovarian tumors from the surface epithelium and should promote heightened interest in the gonadotropin theory of ovarian tumorigenesis. More importantly, this study shows the feasibility of evaluating FSHR expression by both in situ hybridization and reverse transcription polymerase chain reaction. Application of these techniques to tumor specimens will help to elucidate the role of gonadotropins and their receptors in the carcinogenesis of gynecological tumors.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalAmerican Journal of Pathology
Volume148
Issue number1
StatePublished - Jan 1 1996

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FSH Receptors
Fallopian Tubes
Epithelium
Gonadotropin Receptors
Reverse Transcription
Theca Cells
Granulosa Cells
Gonadotropins
Polymerase Chain Reaction
In Situ Hybridization
Ovary
Carcinogenesis
RNA Probes
Neoplasms
Ovarian Follicle
Feasibility Studies
Ovarian Neoplasms
Binding Sites
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Follicle-stimulating hormone receptor is expressed in human ovarian surface epithelium and fallopian tube. / Zheng, Wenxin; Magid, Margret S.; Kramer, Elmer E.; Chen, Yao Tseng.

In: American Journal of Pathology, Vol. 148, No. 1, 01.01.1996, p. 47-53.

Research output: Contribution to journalArticle

Zheng, Wenxin ; Magid, Margret S. ; Kramer, Elmer E. ; Chen, Yao Tseng. / Follicle-stimulating hormone receptor is expressed in human ovarian surface epithelium and fallopian tube. In: American Journal of Pathology. 1996 ; Vol. 148, No. 1. pp. 47-53.
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