Formaldehyde adducts of glutathione. Structure elucidation by two-dimensional n.m.r. spectroscopy and fast-atom-bombardment tandem mass spectrometry.

S. Naylor, R. P. Mason, J. K. Sanders, D. H. Williams, G. Moneti

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Aqueous mixtures of formaldehyde and glutathione react to form a variety of cyclized adducts in addition to S-hydroxymethylglutathione. The adducts are in labile equilibrium with each other and are not readily separated. The structures of two of the other major adducts were determined by concerted application of 13C-1H two-dimensional chemical-shift correlation, fast-atom-bombardment mass spectrometry and tandem mass spectrometry to the adduct mixtures in aqueous solution.

Original languageEnglish (US)
Pages (from-to)573-579
Number of pages7
JournalThe Biochemical journal
Volume249
Issue number2
DOIs
StatePublished - Jan 15 1988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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