Formaldehyde Metabolism by Escherichia coli. Detection by in Vivo 13C NMR Spectroscopy of s-(Hydroxymethyl)glutathione as a Transient Intracellular Intermediate

Ralph P. Mason, Jeremy K M Sanders, Andrew Crawford, Brian K. Hunter

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

In vivo 13C NMR has been used to detect the transient formation of S-(hydroxymethyl)glutathione (GSCH2OH) from glutathione and [13C] formaldehyde in Escherichia coli. Two-dimensional shift 1H-13C correlation was used to locate the chemical shift of the formaldehyde-derived protons of the adduct. The adduct GSCH2OH is formed by chemical reaction in the first few minutes after cells are challenged with formaldehyde and remains within the cell until consumed by metabolism.

Original languageEnglish (US)
Pages (from-to)4504-4507
Number of pages4
JournalBiochemistry
Volume25
Issue number16
DOIs
StatePublished - Aug 1986

ASJC Scopus subject areas

  • Biochemistry

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