Formaldehyde metabolism by escherichia coli. Detection by in Vivo13C NMR spectroscopy of S-(hydroxymethyl)glutathione as a transient intracellular intermediate

Ralph P. Mason, Jeremy K M Sanders, Andrew Crawford, Brian K. Hunter

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In vivo 13C NMR has been used to detect the transient formation of S-(hydroxymethyl)glutathione (GSCH2OH) from glutathione and [13C] formaldehyde in Escherichia coli. Two-dimensional 1H-13C shift correlation was used to locate the chemical shift of the formaldehyde-derived protons of the adduct. The adduct GSCH2OH is formed by chemical reaction in the first few minutes after cells are challenged with formaldehyde and remains within the cell until consumed by metabolism.

Original languageEnglish (US)
Pages (from-to)4504-4507
Number of pages4
JournalBiochemistry®
Volume25
Issue number16
StatePublished - 1986

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Metabolism
Escherichia coli
Formaldehyde
Nuclear magnetic resonance spectroscopy
Glutathione
Magnetic Resonance Spectroscopy
Chemical shift
Protons
Chemical reactions
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Biochemistry

Cite this

Formaldehyde metabolism by escherichia coli. Detection by in Vivo13C NMR spectroscopy of S-(hydroxymethyl)glutathione as a transient intracellular intermediate. / Mason, Ralph P.; Sanders, Jeremy K M; Crawford, Andrew; Hunter, Brian K.

In: Biochemistry®, Vol. 25, No. 16, 1986, p. 4504-4507.

Research output: Contribution to journalArticle

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