Formaldehyde Metabolism by Escherichia coli. Detection by in Vivo 13C NMR Spectroscopy of s-(Hydroxymethyl)glutathione as a Transient Intracellular Intermediate

Ralph P. Mason; Jeremy K M Sanders; Andrew Crawford; Brian K. Hunter

doi:10.1021/bi00364a008

Formaldehyde Metabolism by Escherichia coli. Detection by in Vivo ¹³C NMR Spectroscopy of s-(Hydroxymethyl)glutathione as a Transient Intracellular Intermediate

Ralph P. Mason, Jeremy K M Sanders, Andrew Crawford, Brian K. Hunter

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Abstract

In vivo ¹³C NMR has been used to detect the transient formation of S-(hydroxymethyl)glutathione (GSCH₂OH) from glutathione and [¹³C] formaldehyde in Escherichia coli. Two-dimensional shift ¹H-¹³C correlation was used to locate the chemical shift of the formaldehyde-derived protons of the adduct. The adduct GSCH₂OH is formed by chemical reaction in the first few minutes after cells are challenged with formaldehyde and remains within the cell until consumed by metabolism.

Original language	English (US)
Pages (from-to)	4504-4507
Number of pages	4
Journal	Biochemistry
Volume	25
Issue number	16
DOIs	https://doi.org/10.1021/bi00364a008
State	Published - Aug 1986

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Biochemistry

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abstract = "In vivo 13C NMR has been used to detect the transient formation of S-(hydroxymethyl)glutathione (GSCH2OH) from glutathione and [13C] formaldehyde in Escherichia coli. Two-dimensional shift 1H-13C correlation was used to locate the chemical shift of the formaldehyde-derived protons of the adduct. The adduct GSCH2OH is formed by chemical reaction in the first few minutes after cells are challenged with formaldehyde and remains within the cell until consumed by metabolism.",

author = "Mason, {Ralph P.} and Sanders, {Jeremy K M} and Andrew Crawford and Hunter, {Brian K.}",

year = "1986",

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T1 - Formaldehyde Metabolism by Escherichia coli. Detection by in Vivo 13C NMR Spectroscopy of s-(Hydroxymethyl)glutathione as a Transient Intracellular Intermediate

AU - Mason, Ralph P.

AU - Sanders, Jeremy K M

AU - Crawford, Andrew

AU - Hunter, Brian K.

PY - 1986/8

Y1 - 1986/8

N2 - In vivo 13C NMR has been used to detect the transient formation of S-(hydroxymethyl)glutathione (GSCH2OH) from glutathione and [13C] formaldehyde in Escherichia coli. Two-dimensional shift 1H-13C correlation was used to locate the chemical shift of the formaldehyde-derived protons of the adduct. The adduct GSCH2OH is formed by chemical reaction in the first few minutes after cells are challenged with formaldehyde and remains within the cell until consumed by metabolism.

AB - In vivo 13C NMR has been used to detect the transient formation of S-(hydroxymethyl)glutathione (GSCH2OH) from glutathione and [13C] formaldehyde in Escherichia coli. Two-dimensional shift 1H-13C correlation was used to locate the chemical shift of the formaldehyde-derived protons of the adduct. The adduct GSCH2OH is formed by chemical reaction in the first few minutes after cells are challenged with formaldehyde and remains within the cell until consumed by metabolism.

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Formaldehyde Metabolism by Escherichia coli. Detection by in Vivo ¹³C NMR Spectroscopy of s-(Hydroxymethyl)glutathione as a Transient Intracellular Intermediate

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