Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

Yuan Lin, David S W Protter, Michael K. Rosen, Roy Parker

Research output: Contribution to journalArticle

384 Citations (Scopus)

Abstract

Eukaryotic cells possess numerous dynamic membrane-less organelles, RNP granules, enriched in RNA and RNA-binding proteins containing disordered regions. We demonstrate that the disordered regions of key RNP granule components and the full-length granule protein hnRNPA1 can phase separate in vitro, producing dynamic liquid droplets. Phase separation is promoted by low salt concentrations or RNA. Over time, the droplets mature to more stable states, as assessed by slowed fluorescence recovery after photobleaching and resistance to salt. Maturation often coincides with formation of fibrous structures. Different disordered domains can co-assemble into phase-separated droplets. These biophysical properties demonstrate a plausible mechanism by which interactions between disordered regions, coupled with RNA binding, could contribute to RNP granule assembly in vivo through promoting phase separation. Progression from dynamic liquids to stable fibers may be regulated to produce cellular structures with diverse physiochemical properties and functions. Misregulation could contribute to diseases involving aberrant RNA granules.

Original languageEnglish (US)
Pages (from-to)208-219
Number of pages12
JournalMolecular Cell
Volume60
Issue number2
DOIs
StatePublished - Oct 15 2015

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RNA-Binding Proteins
RNA
Salts
Fluorescence Recovery After Photobleaching
Eukaryotic Cells
Cellular Structures
Organelles
Membranes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins. / Lin, Yuan; Protter, David S W; Rosen, Michael K.; Parker, Roy.

In: Molecular Cell, Vol. 60, No. 2, 15.10.2015, p. 208-219.

Research output: Contribution to journalArticle

Lin, Yuan ; Protter, David S W ; Rosen, Michael K. ; Parker, Roy. / Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins. In: Molecular Cell. 2015 ; Vol. 60, No. 2. pp. 208-219.
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