Formation of 5α-reduced androgens in the testes and urogenital tract of the grey short-tailed opossum, Monodelphis domestica

Jean D. Wilson, Marilyn B. Renfree, Richard J. Auchus, Andrew J. Pask, Geoffrey Shaw

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Testicular 5-reduced androgens, largely 5-androstane-3,17-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with [3H]- progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). The only 19-carbon metabolites identified in the youngest ages (526 days) and the major metabolites in adult testes were testosterone and androstenedione. At 30, 42 and 49 days of age, dihydrotestosterone and small amounts of androstanediol were present. Time-sequence studies indicated that dihydrotestosterone and androstanediol were formed from the 5-reduction (and 3-keto reduction) of testosterone. In a second series of experiments, tissue fragments of a variety of urogenital tract tissues were incubated with [ 3H]-testosterone and the metabolites separated by HPLC. During the interval in which male urogenital tract differentiation takes place in this species (between Days 15 and 28), the major metabolite identified was dihydrotestosterone. We conclude that the timing of 5-reductase expression in the testes of the grey short-tailed possum resembles that of rodents and the brushtail possum rather than that of the tammar wallaby and that dihydrotestosterone is probably the intracellular androgen responsible for virilisation of the urogenital tract in this species.

Original languageEnglish (US)
Pages (from-to)649-654
Number of pages6
JournalReproduction, Fertility and Development
Volume21
Issue number5
DOIs
StatePublished - 2009

Fingerprint

Monodelphis
Monodelphis domestica
Androstane-3,17-diol
Dihydrotestosterone
opossums
androgens
Virilism
Androgens
Testis
testes
masculinization
glycols
metabolites
Macropodidae
Testosterone
Marsupialia
testosterone
Macropus eugenii
Rodentia
Metatheria

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology
  • Reproductive Medicine
  • Animal Science and Zoology

Cite this

Formation of 5α-reduced androgens in the testes and urogenital tract of the grey short-tailed opossum, Monodelphis domestica. / Wilson, Jean D.; Renfree, Marilyn B.; Auchus, Richard J.; Pask, Andrew J.; Shaw, Geoffrey.

In: Reproduction, Fertility and Development, Vol. 21, No. 5, 2009, p. 649-654.

Research output: Contribution to journalArticle

Wilson, Jean D. ; Renfree, Marilyn B. ; Auchus, Richard J. ; Pask, Andrew J. ; Shaw, Geoffrey. / Formation of 5α-reduced androgens in the testes and urogenital tract of the grey short-tailed opossum, Monodelphis domestica. In: Reproduction, Fertility and Development. 2009 ; Vol. 21, No. 5. pp. 649-654.
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