Forskolin and camptothecin induce a 30 kDa protein associated with melatonin production in Y79 human retinoblastoma cells

J. L. Janavs, J. C. Florez, M. E. Pierce, J. S. Takahashi

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The synthesis of melatonin in Xenopus retinas, chick and quail retinal cell cultures, and Y79 human retinoblastoma cells is stimulated by cAMP through a protein synthesis-dependent mechanism. In Y79 retinoblastoma cells, combined treatment with the RNA synthesis inhibitor camptothecin and agents that elevate cAMP, such as forskolin, causes a synergistic elevation of melatonin. Using two-dimensional gel analysis we have identified a 30 kDa cytosolic protein (p30) whose radiolabeling was consistently increased in parallel with increases in arylalkylamine N-acetyltransferase activity and melatonin production that were induced by forskolin and/or camptothecin. Pulse-chase experiments suggest that the elevation in radiolabeling of p30 is due to increased synthesis. Three candidate proteins found in the mammalian pineal, protein 14-3-3, malate dehydrogenase, and recoverin, do not comigrate with p30.

Original languageEnglish (US)
Pages (from-to)298-309
Number of pages12
JournalJournal of Neuroscience
Volume15
Issue number1 I
StatePublished - Jan 1 1995

Keywords

  • N- acetyltransferase
  • RNA synthesis
  • cAMP
  • camptothecin
  • forskolin
  • melatonin
  • protein synthesis
  • retinoblastoma
  • two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Neuroscience(all)

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