Forward genetic analysis of TLR-signaling pathways: An evaluation

Kasper Hoebe, Bruce Beutler

Research output: Contribution to journalReview articlepeer-review

22 Scopus citations


Forward genetic approaches have contributed to our understanding of how the host senses infectious agents such as bacteria, viruses and fungi. Beginning with the initial discovery of Toll-like receptors (TLRs) as primary sensors involved in the recognition of microbial components, our laboratory has taken a forward genetic approach, using N-ethyl-N-nitrosourea (ENU) mutagenesis in mice, to decipher TLR-signaling pathways. This long term effort has helped to elucidate the circuitry of these pathways, identified new molecules, and disclosed new functions for known molecules. Here we review some of the more important insights developed from this approach and discuss its prospects.

Original languageEnglish (US)
Pages (from-to)824-829
Number of pages6
JournalAdvanced Drug Delivery Reviews
Issue number7
StatePublished - Apr 29 2008


  • ENU
  • Innate immunity
  • Mutagenesis
  • TLR

ASJC Scopus subject areas

  • Pharmaceutical Science


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