TY - JOUR
T1 - Forward genetic analysis of TLR-signaling pathways
T2 - An evaluation
AU - Hoebe, Kasper
AU - Beutler, Bruce
PY - 2008/4/29
Y1 - 2008/4/29
N2 - Forward genetic approaches have contributed to our understanding of how the host senses infectious agents such as bacteria, viruses and fungi. Beginning with the initial discovery of Toll-like receptors (TLRs) as primary sensors involved in the recognition of microbial components, our laboratory has taken a forward genetic approach, using N-ethyl-N-nitrosourea (ENU) mutagenesis in mice, to decipher TLR-signaling pathways. This long term effort has helped to elucidate the circuitry of these pathways, identified new molecules, and disclosed new functions for known molecules. Here we review some of the more important insights developed from this approach and discuss its prospects.
AB - Forward genetic approaches have contributed to our understanding of how the host senses infectious agents such as bacteria, viruses and fungi. Beginning with the initial discovery of Toll-like receptors (TLRs) as primary sensors involved in the recognition of microbial components, our laboratory has taken a forward genetic approach, using N-ethyl-N-nitrosourea (ENU) mutagenesis in mice, to decipher TLR-signaling pathways. This long term effort has helped to elucidate the circuitry of these pathways, identified new molecules, and disclosed new functions for known molecules. Here we review some of the more important insights developed from this approach and discuss its prospects.
KW - ENU
KW - Innate immunity
KW - Mutagenesis
KW - TLR
UR - http://www.scopus.com/inward/record.url?scp=41349100214&partnerID=8YFLogxK
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U2 - 10.1016/j.addr.2008.02.002
DO - 10.1016/j.addr.2008.02.002
M3 - Review article
C2 - 18331766
AN - SCOPUS:41349100214
SN - 0169-409X
VL - 60
SP - 824
EP - 829
JO - Advanced Drug Delivery Reviews
JF - Advanced Drug Delivery Reviews
IS - 7
ER -