TY - JOUR
T1 - FOXP3 defines regulatory t cells in human tumor and autoimmune disease
AU - Kryczek, Ilona
AU - Liu, Rebecca
AU - Wang, Guobin
AU - Wu, Ke
AU - Shu, Xiaogong
AU - Szeliga, Wojciech
AU - Vatan, Linhua
AU - Finlayson, Emily
AU - Huang, Emina
AU - Simeone, Diane
AU - Redman, Bruce
AU - Welling, Theodore H.
AU - Chang, Alfred
AU - Zou, Weiping
PY - 2009/5/1
Y1 - 2009/5/1
N2 - Activated T cells may express FOXP3. It is thought that FOXP3 is not a specific marker to determine regulatory T cells (Treg) in humans. Here, we examined the functional phenotype and cytokine profile of the in vitro induced FOXP3 + T cells, primary FOXP3 + and FOXP3- T cells in patients with ulcerative colitis and tumors including colon carcinoma, melanoma, hepatic carcinoma, ovarian carcinoma, pancreatic cancer, and renal cell carcinoma. We observed similar levels of suppressive capacity of primary FOXP3 + T cells in blood, tumors, and colitic tissues. Compared with primary FOXP3 - T cells in the same microenvironment, these primary FOXP3 + T cells expressed minimal levels of effector cytokines, negligible amount of cytotoxic molecule granzyme B, and levels of suppressive molecules interleukin-10 and PD-1. Although the in vitro + activated T cells expressed FOXP3, these induced FOXP3 + T cells expressed high levels of multiple effector cytokines and were not functionally suppressive. The data reinforce the fact that FOXP3 remains an accurate marker to define primary Tregs in patients with cancer and autoimmune disease. We suggest that the combination of FOXP3 and cytokine profile is useful for further functionally distinguishing primary Tregs from activated conventional T cells.
AB - Activated T cells may express FOXP3. It is thought that FOXP3 is not a specific marker to determine regulatory T cells (Treg) in humans. Here, we examined the functional phenotype and cytokine profile of the in vitro induced FOXP3 + T cells, primary FOXP3 + and FOXP3- T cells in patients with ulcerative colitis and tumors including colon carcinoma, melanoma, hepatic carcinoma, ovarian carcinoma, pancreatic cancer, and renal cell carcinoma. We observed similar levels of suppressive capacity of primary FOXP3 + T cells in blood, tumors, and colitic tissues. Compared with primary FOXP3 - T cells in the same microenvironment, these primary FOXP3 + T cells expressed minimal levels of effector cytokines, negligible amount of cytotoxic molecule granzyme B, and levels of suppressive molecules interleukin-10 and PD-1. Although the in vitro + activated T cells expressed FOXP3, these induced FOXP3 + T cells expressed high levels of multiple effector cytokines and were not functionally suppressive. The data reinforce the fact that FOXP3 remains an accurate marker to define primary Tregs in patients with cancer and autoimmune disease. We suggest that the combination of FOXP3 and cytokine profile is useful for further functionally distinguishing primary Tregs from activated conventional T cells.
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U2 - 10.1158/0008-5472.CAN-08-3804
DO - 10.1158/0008-5472.CAN-08-3804
M3 - Article
C2 - 19383912
AN - SCOPUS:65949111746
SN - 0008-5472
VL - 69
SP - 3995
EP - 4000
JO - Cancer research
JF - Cancer research
IS - 9
ER -