Fracture healing in protease-activated receptor-2 deficient mice

Kevin R. O'Neill, Christopher M. Stutz, Nicholas A. Mignemi, Heather Cole, Matthew R. Murry, Jeffry S. Nyman, Heidi Hamm, Jonathan G. Schoenecker

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Protease-activated receptor-2 (PAR-2) provides an important link between extracellular proteases and the cellular initiation of inflammatory responses. The effect of PAR-2 on fracture healing is unknown. This study investigates the in vivo effect of PAR-2 deletion on fracture healing by assessing differences between wild-type (PAR-2 +/+) and knock-out (PAR-2 -/-) mice. Unilateral mid-shaft femur fractures were created in 34 PAR-2 +/+ and 28 PAR-2 -/- mice after intramedullary fixation. Histologic assessments were made at 1, 2, and 4 weeks post-fracture (wpf), and radiographic (plain radiographs, micro-computed tomography (μCT)) and biomechanical (torsion testing) assessments were made at 7 and 10 wpf. Both the fractured and un-fractured contralateral femur specimens were evaluated. Polar moment of inertia (pMOI), tissue mineral density (TMD), bone volume fraction (BV/TV) were determined from μCT images, and callus diameter was determined from plain radiographs. Statistically significant differences in callus morphology as assessed by μCT were found between PAR-2 -/- and PAR-2 +/+ mice at both 7 and 10 wpf. However, no significant histologic, plain radiographic, or biomechanical differences were found between the genotypes. The loss of PAR-2 was found to alter callus morphology as assessed by μCT but was not found to otherwise effect fracture healing in young mice.

Original languageEnglish (US)
Pages (from-to)1271-1276
Number of pages6
JournalJournal of Orthopaedic Research
Volume30
Issue number8
DOIs
StatePublished - Aug 1 2012

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PAR-2 Receptor
Fracture Healing
Bony Callus
Tomography
Femur
Bone Density
Peptide Hydrolases
Genotype

Keywords

  • bone biology
  • fracture
  • inflammation
  • mice
  • protease-activated receptor

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

O'Neill, K. R., Stutz, C. M., Mignemi, N. A., Cole, H., Murry, M. R., Nyman, J. S., ... Schoenecker, J. G. (2012). Fracture healing in protease-activated receptor-2 deficient mice. Journal of Orthopaedic Research, 30(8), 1271-1276. https://doi.org/10.1002/jor.22071

Fracture healing in protease-activated receptor-2 deficient mice. / O'Neill, Kevin R.; Stutz, Christopher M.; Mignemi, Nicholas A.; Cole, Heather; Murry, Matthew R.; Nyman, Jeffry S.; Hamm, Heidi; Schoenecker, Jonathan G.

In: Journal of Orthopaedic Research, Vol. 30, No. 8, 01.08.2012, p. 1271-1276.

Research output: Contribution to journalArticle

O'Neill, KR, Stutz, CM, Mignemi, NA, Cole, H, Murry, MR, Nyman, JS, Hamm, H & Schoenecker, JG 2012, 'Fracture healing in protease-activated receptor-2 deficient mice', Journal of Orthopaedic Research, vol. 30, no. 8, pp. 1271-1276. https://doi.org/10.1002/jor.22071
O'Neill, Kevin R. ; Stutz, Christopher M. ; Mignemi, Nicholas A. ; Cole, Heather ; Murry, Matthew R. ; Nyman, Jeffry S. ; Hamm, Heidi ; Schoenecker, Jonathan G. / Fracture healing in protease-activated receptor-2 deficient mice. In: Journal of Orthopaedic Research. 2012 ; Vol. 30, No. 8. pp. 1271-1276.
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