Fragile histidine triad gene abnormalities in the pathogenesis of gallbladder carcinoma

Ignacio I. Wistuba, Raheela Ashfaq, Anirban Maitra, Hector Alvarez, Erick Riquelme, Adi F. Gazdar

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Abstract

There is limited information about the molecular changes involved in the pathogenesis of gallbladder carcinoma (GBC). Our recent allelotyping analyses have indicated that chromosome 3p loss of heterozygosity (LOH), including the fragile histidine triad (FHIT) candidate tumor-suppressor gene locus at 3p14.2, is frequently detected in this neoplasm. To investigate the role of the FHIT abnormalities in the multistage sequential development of GBC, 33 formalin-fixed paraffin-embedded invasive GBC specimens and 76 accompanying histologically normal (n = 43) and dysplastic (n = 33) epithelia were examined by immunostaining for expression of Fhit protein. Allele loss at the FHIT gene locus (3p14.2) was studied in all GBCs and in a subset of accompanying gallbladder epithelia by polymerase chain reaction-based LOH analysis, using three 3p14.2 microsatellite markers. In addition, histologically normal epithelium from chronic cholecystitis (n = 19) and dysplasia (n = 13) from gallbladder specimens without cancer were examined for immunostaining and LOH. There was a progressive increase in both the frequency of loss of Fhit expression and LOH at FHIT with increasing severity of histopathological changes. FHIT abnormalities were occasionally demonstrated in histologically normal gallbladder epithelium. Dysplastic foci demonstrated frequent reduction or absence of Fhit immunostaining (38 to 55%) and FHIT allelic loss (33 to 46%). In invasive tumors, these abnormalities were even higher, with 79% reduction or absence of Fhit immunostaining and 76% FHIT allele loss. A high correlation (70%) was observed between Fhit immunostaining abnormalities and allele loss in GBC specimens (P < 0.05). Although a high frequency of FHIT locus breakpoints were detected in both invasive and dysplastic gallbladder specimens, no intronic homozygous deletions on FHIT were detected in GBCs. FHIT gene abnormalities are nearly universal in GBC and these changes are detected early in the sequential development of this neoplasm. Our findings indicate that the FHIT gene is one of the chromosome 3p putative tumor suppressor genes involved in the pathogenesis of this highly malignant neoplasm.

Original languageEnglish (US)
Pages (from-to)2073-2079
Number of pages7
JournalAmerican Journal of Pathology
Volume160
Issue number6
StatePublished - 2002

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Gallbladder
Histidine
Carcinoma
Loss of Heterozygosity
Genes
Epithelium
Alleles
Neoplasms
Tumor Suppressor Genes
Chromosomes
Cholecystitis
Paraffin
Microsatellite Repeats
Formaldehyde
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Wistuba, I. I., Ashfaq, R., Maitra, A., Alvarez, H., Riquelme, E., & Gazdar, A. F. (2002). Fragile histidine triad gene abnormalities in the pathogenesis of gallbladder carcinoma. American Journal of Pathology, 160(6), 2073-2079.

Fragile histidine triad gene abnormalities in the pathogenesis of gallbladder carcinoma. / Wistuba, Ignacio I.; Ashfaq, Raheela; Maitra, Anirban; Alvarez, Hector; Riquelme, Erick; Gazdar, Adi F.

In: American Journal of Pathology, Vol. 160, No. 6, 2002, p. 2073-2079.

Research output: Contribution to journalArticle

Wistuba, II, Ashfaq, R, Maitra, A, Alvarez, H, Riquelme, E & Gazdar, AF 2002, 'Fragile histidine triad gene abnormalities in the pathogenesis of gallbladder carcinoma', American Journal of Pathology, vol. 160, no. 6, pp. 2073-2079.
Wistuba, Ignacio I. ; Ashfaq, Raheela ; Maitra, Anirban ; Alvarez, Hector ; Riquelme, Erick ; Gazdar, Adi F. / Fragile histidine triad gene abnormalities in the pathogenesis of gallbladder carcinoma. In: American Journal of Pathology. 2002 ; Vol. 160, No. 6. pp. 2073-2079.
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abstract = "There is limited information about the molecular changes involved in the pathogenesis of gallbladder carcinoma (GBC). Our recent allelotyping analyses have indicated that chromosome 3p loss of heterozygosity (LOH), including the fragile histidine triad (FHIT) candidate tumor-suppressor gene locus at 3p14.2, is frequently detected in this neoplasm. To investigate the role of the FHIT abnormalities in the multistage sequential development of GBC, 33 formalin-fixed paraffin-embedded invasive GBC specimens and 76 accompanying histologically normal (n = 43) and dysplastic (n = 33) epithelia were examined by immunostaining for expression of Fhit protein. Allele loss at the FHIT gene locus (3p14.2) was studied in all GBCs and in a subset of accompanying gallbladder epithelia by polymerase chain reaction-based LOH analysis, using three 3p14.2 microsatellite markers. In addition, histologically normal epithelium from chronic cholecystitis (n = 19) and dysplasia (n = 13) from gallbladder specimens without cancer were examined for immunostaining and LOH. There was a progressive increase in both the frequency of loss of Fhit expression and LOH at FHIT with increasing severity of histopathological changes. FHIT abnormalities were occasionally demonstrated in histologically normal gallbladder epithelium. Dysplastic foci demonstrated frequent reduction or absence of Fhit immunostaining (38 to 55{\%}) and FHIT allelic loss (33 to 46{\%}). In invasive tumors, these abnormalities were even higher, with 79{\%} reduction or absence of Fhit immunostaining and 76{\%} FHIT allele loss. A high correlation (70{\%}) was observed between Fhit immunostaining abnormalities and allele loss in GBC specimens (P < 0.05). Although a high frequency of FHIT locus breakpoints were detected in both invasive and dysplastic gallbladder specimens, no intronic homozygous deletions on FHIT were detected in GBCs. FHIT gene abnormalities are nearly universal in GBC and these changes are detected early in the sequential development of this neoplasm. Our findings indicate that the FHIT gene is one of the chromosome 3p putative tumor suppressor genes involved in the pathogenesis of this highly malignant neoplasm.",
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