Fragility in the 14q21q translocation region

Stacy R. Denison, Asha S. Multani, Sen Pathak, Ira F. Greenbaum

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of β-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes, or that this fragility was the result of the fusion of nonfragile progenitor chromosomes.

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalGenetics and Molecular Biology
Volume25
Issue number3
StatePublished - Sep 2002

Fingerprint

Aphidicolin
Chromosome Breakage
Chromosome Fragility
Chromosomes
Constitution and Bylaws
Metaphase
Fluorescence In Situ Hybridization
Lymphocytes
Mutation

Keywords

  • Aphidicolin
  • Fragile sites
  • Heritability
  • Rob(14q21q)
  • Robertsonian translocation

ASJC Scopus subject areas

  • Genetics

Cite this

Denison, S. R., Multani, A. S., Pathak, S., & Greenbaum, I. F. (2002). Fragility in the 14q21q translocation region. Genetics and Molecular Biology, 25(3), 271-276.

Fragility in the 14q21q translocation region. / Denison, Stacy R.; Multani, Asha S.; Pathak, Sen; Greenbaum, Ira F.

In: Genetics and Molecular Biology, Vol. 25, No. 3, 09.2002, p. 271-276.

Research output: Contribution to journalArticle

Denison, SR, Multani, AS, Pathak, S & Greenbaum, IF 2002, 'Fragility in the 14q21q translocation region', Genetics and Molecular Biology, vol. 25, no. 3, pp. 271-276.
Denison SR, Multani AS, Pathak S, Greenbaum IF. Fragility in the 14q21q translocation region. Genetics and Molecular Biology. 2002 Sep;25(3):271-276.
Denison, Stacy R. ; Multani, Asha S. ; Pathak, Sen ; Greenbaum, Ira F. / Fragility in the 14q21q translocation region. In: Genetics and Molecular Biology. 2002 ; Vol. 25, No. 3. pp. 271-276.
@article{c6e861e271a943e9b6134f4c9754631c,
title = "Fragility in the 14q21q translocation region",
abstract = "Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of β-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes, or that this fragility was the result of the fusion of nonfragile progenitor chromosomes.",
keywords = "Aphidicolin, Fragile sites, Heritability, Rob(14q21q), Robertsonian translocation",
author = "Denison, {Stacy R.} and Multani, {Asha S.} and Sen Pathak and Greenbaum, {Ira F.}",
year = "2002",
month = "9",
language = "English (US)",
volume = "25",
pages = "271--276",
journal = "Genetics and Molecular Biology",
issn = "1415-4757",
publisher = "Brazilian Society of Genetics",
number = "3",

}

TY - JOUR

T1 - Fragility in the 14q21q translocation region

AU - Denison, Stacy R.

AU - Multani, Asha S.

AU - Pathak, Sen

AU - Greenbaum, Ira F.

PY - 2002/9

Y1 - 2002/9

N2 - Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of β-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes, or that this fragility was the result of the fusion of nonfragile progenitor chromosomes.

AB - Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of β-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes, or that this fragility was the result of the fusion of nonfragile progenitor chromosomes.

KW - Aphidicolin

KW - Fragile sites

KW - Heritability

KW - Rob(14q21q)

KW - Robertsonian translocation

UR - http://www.scopus.com/inward/record.url?scp=0036760679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036760679&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 271

EP - 276

JO - Genetics and Molecular Biology

JF - Genetics and Molecular Biology

SN - 1415-4757

IS - 3

ER -