FTT0831c/FTL_0325 contributes to Francisella tularensis cell division, maintenance of cell shape, and structural integrity

Gregory T. Robertson, Elizabeth Di Russo Case, Nicole Dobbs, Christine Ingle, Murat Balaban, Jean Celli, Michael V. Norgard

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The Francisella FTT0831c/FTL_0325 gene encodes amino acid motifs to suggest it is a lipoprotein and that it may interact with the bacterial cell wall as a member of the OmpA-like protein family. Previous studies have suggested that FTT0831c is surface exposed and required for virulence of Francisella tularensis by subverting the host innate immune response (M. Mahawar et al., J. Biol. Chem. 287:25216-25229, 2012). We also found that FTT0831c is required for murine pathogenesis and intramacrophage growth of Schu S4, but we propose a different model to account for the proinflammatory nature of the resultant mutants. First, inactivation of FTL_0325 from live vaccine strain (LVS) or FTT0831c from Schu S4 resulted in temperature-dependent defects in cell viability and morphology. Loss of FTT0831c was also associated with an unusual defect in lipopolysaccharide O-antigen synthesis, but loss of FTL_0325 was not. Full restoration of these properties was observed in complemented strains expressing FTT0831c in trans, but not in strains lacking the OmpA motif, suggesting that cell wall contact is required. Finally, growth of the LVS FTL_0325 mutant in Mueller-Hinton broth at 37°C resulted in the appearance of membrane blebs at the poles and midpoint, prior to the formation of enlarged round cells that showed evidence of compromised cellular membranes. Taken together, these data are more consistent with the known structural role of OmpA-like proteins in linking theOMto the cell wall and, as such, maintenance of structural integrity preventing altered surface exposure or release of Toll-like receptor 2 agonists during rapid growth of Francisella in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)2935-2948
Number of pages14
JournalInfection and Immunity
Volume82
Issue number7
DOIs
StatePublished - 2014

Fingerprint

Francisella tularensis
Cell Shape
Francisella
Cell Division
Cell Wall
Maintenance
Vaccines
Growth
Toll-Like Receptor 2
O Antigens
Amino Acid Motifs
Membranes
Blister
Innate Immunity
Lipoproteins
Virulence
Lipopolysaccharides
Cell Survival
Temperature
Genes

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

FTT0831c/FTL_0325 contributes to Francisella tularensis cell division, maintenance of cell shape, and structural integrity. / Robertson, Gregory T.; Russo Case, Elizabeth Di; Dobbs, Nicole; Ingle, Christine; Balaban, Murat; Celli, Jean; Norgard, Michael V.

In: Infection and Immunity, Vol. 82, No. 7, 2014, p. 2935-2948.

Research output: Contribution to journalArticle

Robertson, Gregory T. ; Russo Case, Elizabeth Di ; Dobbs, Nicole ; Ingle, Christine ; Balaban, Murat ; Celli, Jean ; Norgard, Michael V. / FTT0831c/FTL_0325 contributes to Francisella tularensis cell division, maintenance of cell shape, and structural integrity. In: Infection and Immunity. 2014 ; Vol. 82, No. 7. pp. 2935-2948.
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AU - Norgard, Michael V.

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