Full-field time domain optical molecular imaging system

David J. Hall, David R. Vera, Robert F. Mattrey

Research output: Contribution to journalConference article

5 Scopus citations

Abstract

The advent of optical molecular probes has taken optical imaging beyond approaches limited to intrinsic optical contrast mechanisms. Fluorophores are typically used as the source of contrast for optical molecular probes and the field of optical molecular imaging is concerned with measuring and quantifying their in vivo biodistribution and pharmacokinetics. Most optical molecular imaging systems are based on Continuous Wave (CW) approaches which enable rapid, full-body imaging of small animals and readily yield images of probe location, however quantification of probe concentration is challenging. Time Domain (TD) approaches, although more expensive and complicated than CW, provide more information to assist in determining the probe location and concentration. Moreover, the TD approach permits access to measuring the fluorophore lifetime which can be indicative of the probe's environment. Existing TD approaches involve a point source and detector which are sequentially scanned over the sample and can take several minutes to acquire the data compared to the rapid imaging offered by CW. The system presented here employs a high power, near infrared, pulsed laser to provide area illumination and a temporally gated intensified CCD camera to permit area detection in order to enable rapid, full-body, TD optical molecular imaging of small animals in vivo. The system is described and preliminary in vitro and in vivo measurements are presented.

Original languageEnglish (US)
Article number63
Pages (from-to)330-335
Number of pages6
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5693
DOIs
StatePublished - Aug 16 2005
EventOptical Tomography and Spectroscopy of Tissue VI - San Jose, CA, United States
Duration: Jan 23 2005Jan 26 2005

Keywords

  • Fluorescence
  • In vivo
  • Molecular imaging
  • Near infrared
  • Optical imaging
  • Time domain

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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