Full-length structure of a monomeric histidine kinase reveals basis for sensory regulation

Giomar Rivera-Cancel, Wen Huang Ko, Diana R. Tomchick, Fernando Correa, Kevin H. Gardner

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Although histidine kinases (HKs) are critical sensors of external stimuli in prokaryotes, the mechanisms by which their sensor domains control enzymatic activity remain unclear. Here, we report the full-length structure of a blue light-activated HK from Erythrobacter litoralis HTCC2594 (EL346) and the results of biochemical and biophysical studies that explain how it is activated by light. Contrary to the standard view that signaling occurs within HK dimers, EL346 functions as a monomer. Its structure reveals that the light-oxygen-voltage (LOV) sensor domain both controls kinase activity and prevents dimerization by binding one side of a dimerization/histidine phosphotransfer-like (DHpL) domain. The DHpL domain also contacts the catalytic/ATP-binding (CA) domain, keeping EL346 in an inhibited conformation in the dark. Upon light stimulation, interdomain interactions weaken to facilitate activation. Our data suggest that the LOV domain controls kinase activity by affecting the stability of the DHpL/CA interface, releasing the CA domain from an inhibited conformation upon photoactivation. We suggest parallels between EL346 and dimeric HKs, with sensor-induced movements in the DHp similarly remodeling the DHp/CA interface as part of activation.

Original languageEnglish (US)
Pages (from-to)17839-17844
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number50
DOIs
StatePublished - Dec 16 2014

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Dimerization
Light
Adenosine Triphosphate
Histidine
Sphingomonadaceae
Phosphotransferases
Oxygen
Histidine Kinase

Keywords

  • Cell signaling
  • Histidine kinase
  • Photosensory
  • Regulation
  • Two-component system

ASJC Scopus subject areas

  • General

Cite this

Full-length structure of a monomeric histidine kinase reveals basis for sensory regulation. / Rivera-Cancel, Giomar; Ko, Wen Huang; Tomchick, Diana R.; Correa, Fernando; Gardner, Kevin H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 50, 16.12.2014, p. 17839-17844.

Research output: Contribution to journalArticle

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