Functional characterization of cells separated from suspensions of Hodgkin disease tumor cells in an isokinetic gradient

J. K V Willson, J. L. Zaremba, T. G. Pretlow

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Suspensions of cells from Hodgkin disease tumors were separated by velocity sedimentation in an isokinetic Ficoll gradient. The separated cells were cultured with mitogens. The modal population of lymphocytes from the tumor contained 50%-75% of all lymphocytes separated from the tumor of each studied patient. Despite the fact that these cells appeared to be mature lymphocytes, the majority of which formed nonimmune E rosettes with sheep red blood cells, they showed a lack of responsiveness to phytohemagglutinin which was almost complete in all but one patient. Lymphocytes which had been obtained in suspension and purified by identical methods from tonsils and spleens without tumors did respond to stimulation by phytohemagglutinin.

Original languageEnglish (US)
Pages (from-to)783-797
Number of pages15
JournalBlood
Volume50
Issue number5
StatePublished - 1977

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Lymphocytes
Hodgkin Disease
Tumors
Suspensions
Cells
Phytohemagglutinins
Neoplasms
Ficoll
Palatine Tonsil
Mitogens
Sedimentation
Cultured Cells
Sheep
Blood
Spleen
Erythrocytes
Population

ASJC Scopus subject areas

  • Hematology

Cite this

Functional characterization of cells separated from suspensions of Hodgkin disease tumor cells in an isokinetic gradient. / Willson, J. K V; Zaremba, J. L.; Pretlow, T. G.

In: Blood, Vol. 50, No. 5, 1977, p. 783-797.

Research output: Contribution to journalArticle

Willson, J. K V ; Zaremba, J. L. ; Pretlow, T. G. / Functional characterization of cells separated from suspensions of Hodgkin disease tumor cells in an isokinetic gradient. In: Blood. 1977 ; Vol. 50, No. 5. pp. 783-797.
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