Functional clues from the crystal structure of an orphan periplasmic ligand-binding protein from Treponema pallidum

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The spirochete Treponema pallidum is the causative agent of syphilis, a sexually transmitted infection of major global importance. Other closely related subspecies of Treponema also are the etiological agents of the endemic treponematoses, such as yaws, pinta, and bejel. The inability of T. pallidum and its close relatives to be cultured in vitro has prompted efforts to characterize T. pallidum's proteins structurally and biophysically, particularly those potentially relevant to treponemal membrane biology, with the goal of possibly revealing the functions of those proteins. This report describes the structure of the treponemal protein Tp0737; this polypeptide has a fold characteristic of a class of periplasmic ligand-binding proteins associated with ABC-type transporters. Although no ligand for the protein was observed in electron-density maps, and thus the nature of the native ligand remains obscure, the structural data described herein provide a foundation for further efforts to elucidate the ligand and thus the function of this protein in T. pallidum.

Original languageEnglish (US)
Pages (from-to)847-856
Number of pages10
JournalProtein Science
Volume26
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Periplasmic Binding Proteins
Treponema pallidum
Carrier Proteins
Crystal structure
Ligands
Proteins
Pinta
Treponemal Infections
Treponema
Yaws
Spirochaetales
Globus Pallidus
ATP-Binding Cassette Transporters
Syphilis
Sexually Transmitted Diseases
Carrier concentration
Electrons
Membranes
Peptides

Keywords

  • ABC transporter
  • analytical ultracentrifugation
  • differential scanning fluorimetry
  • syphilis
  • X-ray crystallography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Functional clues from the crystal structure of an orphan periplasmic ligand-binding protein from Treponema pallidum. / Brautigam, Chad A; Deka, Ranjit K.; Liu, Wei Z.; Tomchick, Diana R; Norgard, Michael V.

In: Protein Science, Vol. 26, No. 4, 01.04.2017, p. 847-856.

Research output: Contribution to journalArticle

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