Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5

Murray A. Stackhouse, Jeanelle B. Ortiz, Koki Sato, David J. Chen

Research output: Contribution to journalArticle

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Abstract

The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific primers and fluorescence in situ hybridization. From this data we conclude that the gene that corrects the radiation hypersensitivity of M10 cells is located on chromosome 5 and tentatively assigned to the 5q14 to 5pter region. We designate this gene XRCC4L.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalMutation Research Letters
Volume323
Issue number1-2
DOIs
StatePublished - Jan 1 1994

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Keywords

  • Chromosome mapping
  • DNA double-strand break repair
  • Microcell-mediated chromosome transfer
  • Radiation-sensitive mutants
  • XRCC genes

ASJC Scopus subject areas

  • Medicine(all)

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