Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5

Murray A. Stackhouse; Jeanelle B. Ortiz; Koki Sato; David J. Chen

doi:10.1016/0165-7992(94)90044-2

Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5

Murray A. Stackhouse, Jeanelle B. Ortiz, Koki Sato, David J. Chen

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific primers and fluorescence in situ hybridization. From this data we conclude that the gene that corrects the radiation hypersensitivity of M10 cells is located on chromosome 5 and tentatively assigned to the 5q14 to 5pter region. We designate this gene XRCC4L.

Original language	English (US)
Pages (from-to)	47-52
Number of pages	6
Journal	Mutation Research Letters
Volume	323
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-7992(94)90044-2
State	Published - 1994

Keywords

Chromosome mapping
DNA double-strand break repair
Microcell-mediated chromosome transfer
Radiation-sensitive mutants
XRCC genes

ASJC Scopus subject areas

General Medicine

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10.1016/0165-7992(94)90044-2

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@article{921e5674f6884a13b5e67d999b3aa9b5,

title = "Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5",

abstract = "The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific primers and fluorescence in situ hybridization. From this data we conclude that the gene that corrects the radiation hypersensitivity of M10 cells is located on chromosome 5 and tentatively assigned to the 5q14 to 5pter region. We designate this gene XRCC4L.",

keywords = "Chromosome mapping, DNA double-strand break repair, Microcell-mediated chromosome transfer, Radiation-sensitive mutants, XRCC genes",

author = "Stackhouse, {Murray A.} and Ortiz, {Jeanelle B.} and Koki Sato and Chen, {David J.}",

note = "Funding Information: We thank Mitsuo Oshimuraf or theA 9 micro-cell hybrids and Joel Bedford for the Sendai virus. This work was performedu nderthe aus-piceso f the U.S. Departmenotf Energy Contract KP0400/005181t o the Los Alamos National LaboratoryN, IH Grant CA50519t o D.J.C.",

year = "1994",

doi = "10.1016/0165-7992(94)90044-2",

language = "English (US)",

volume = "323",

pages = "47--52",

journal = "Mutation Research Letters",

issn = "0165-7992",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5

AU - Stackhouse, Murray A.

AU - Ortiz, Jeanelle B.

AU - Sato, Koki

AU - Chen, David J.

N1 - Funding Information: We thank Mitsuo Oshimuraf or theA 9 micro-cell hybrids and Joel Bedford for the Sendai virus. This work was performedu nderthe aus-piceso f the U.S. Departmenotf Energy Contract KP0400/005181t o the Los Alamos National LaboratoryN, IH Grant CA50519t o D.J.C.

PY - 1994

Y1 - 1994

N2 - The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific primers and fluorescence in situ hybridization. From this data we conclude that the gene that corrects the radiation hypersensitivity of M10 cells is located on chromosome 5 and tentatively assigned to the 5q14 to 5pter region. We designate this gene XRCC4L.

AB - The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific primers and fluorescence in situ hybridization. From this data we conclude that the gene that corrects the radiation hypersensitivity of M10 cells is located on chromosome 5 and tentatively assigned to the 5q14 to 5pter region. We designate this gene XRCC4L.

KW - Chromosome mapping

KW - DNA double-strand break repair

KW - Microcell-mediated chromosome transfer

KW - Radiation-sensitive mutants

KW - XRCC genes

UR - http://www.scopus.com/inward/record.url?scp=0028179649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028179649&partnerID=8YFLogxK

U2 - 10.1016/0165-7992(94)90044-2

DO - 10.1016/0165-7992(94)90044-2

M3 - Article

C2 - 7508566

AN - SCOPUS:0028179649

SN - 0165-7992

VL - 323

SP - 47

EP - 52

JO - Mutation Research Letters

JF - Mutation Research Letters

IS - 1-2

ER -

Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5

Abstract

Keywords

ASJC Scopus subject areas

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