Functional disturbances in the striatum by region-specific ablation of NMDA receptors

Nobuhisa Ohtsuka, Morris F. Tansky, Hui Kuang, Saïd Kourrich, Mark J. Thomas, John L R Rubenstein, Marc Ekker, Susan E. Leeman, Joe Z. Tsien

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

To study the role of NMDA receptors in dopamine signaling of the striatum, the brain area that receives glutamatergic inputs from various cortical areas and most dopaminergic inputs, we generated striatum-specific NMDA receptor-deficient mice. The mutant pups showed reduced food intake and retarded growth starting at the second postnatal week and died on approximately postnatal day 20 (P20). The time course of postnatal lethality is similar to that of compound mutant, double knockout of dopamine D1/D2 receptors, or genetically engineered dopamine-deficient mouse. In vivo electrophysiological recordings in the mutant pups showed that frequencies in the range of gamma oscillation were reduced in the striatal circuits. Moreover, the number of functional dopamine receptors in the striatum as measured by D1- and D2-binding experiments was greatly diminished in the mutants as compared with control animals. A consequence of diminished dopamine binding in the striatum manifested in an increase of locomotor activity. The administration of D1/D2 agonists paradoxically reduced the hyperactivity of the mutant mice as compared with an increase in locomotor activity in control mice. These results demonstrate that the NMDA receptor plays an essential role in the integration of dopamine signaling in the striatum and that is required in behavioral function.

Original languageEnglish (US)
Pages (from-to)12961-12966
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number35
DOIs
StatePublished - Sep 2 2008

Fingerprint

N-Methyl-D-Aspartate Receptors
Dopamine
Locomotion
Corpus Striatum
Dopamine D1 Receptors
Dopamine D2 Receptors
Dopamine Receptors
Eating
Brain
Growth

Keywords

  • Dopamine
  • Knockout
  • Locomotion
  • NR1
  • Striatum

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Functional disturbances in the striatum by region-specific ablation of NMDA receptors. / Ohtsuka, Nobuhisa; Tansky, Morris F.; Kuang, Hui; Kourrich, Saïd; Thomas, Mark J.; Rubenstein, John L R; Ekker, Marc; Leeman, Susan E.; Tsien, Joe Z.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 35, 02.09.2008, p. 12961-12966.

Research output: Contribution to journalArticle

Ohtsuka, Nobuhisa ; Tansky, Morris F. ; Kuang, Hui ; Kourrich, Saïd ; Thomas, Mark J. ; Rubenstein, John L R ; Ekker, Marc ; Leeman, Susan E. ; Tsien, Joe Z. / Functional disturbances in the striatum by region-specific ablation of NMDA receptors. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 35. pp. 12961-12966.
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