Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation

Zhaogang Yang, Jing Xie, Jing Zhu, Chen Kang, Chiling Chiang, Xinmei Wang, Xiaobing Wang, Tairong Kuang, Feng Chen, Zhou Chen, Aili Zhang, Bo Yu, Robert J. Lee, Lesheng Teng, L. James Lee

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel strategy to produce nanoscale exosome-mimics (EMs) in sufficient quantity for gene delivery in cancer both in vitro and in vivo. Size-controllable EMs were generated at a high yield by serial extrusion of non-tumorigenic epithelial MCF-10A cells through filters with different pore sizes. siRNA was then encapsulated into the EMs by electroporation. Biosafety and uptake efficiency of the EMs were evaluated both in vitro and in vivo. The mechanism underlying their cellular endocytosis was also studied.

Original languageEnglish (US)
Pages (from-to)160-171
Number of pages12
JournalJournal of Controlled Release
Volume243
DOIs
StatePublished - Dec 10 2016
Externally publishedYes

    Fingerprint

Keywords

  • Electroporation
  • Endocytosis
  • Exosome
  • MCF-7
  • siRNA

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Yang, Z., Xie, J., Zhu, J., Kang, C., Chiang, C., Wang, X., Wang, X., Kuang, T., Chen, F., Chen, Z., Zhang, A., Yu, B., Lee, R. J., Teng, L., & Lee, L. J. (2016). Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation. Journal of Controlled Release, 243, 160-171. https://doi.org/10.1016/j.jconrel.2016.10.008