Functional genomic analysis of cell division by endoribonuclease-prepared siRNAs

Ralf Kittler, Frank Buchholz

Research output: Contribution to journalReview article

30 Scopus citations

Abstract

The emergence of RNA interference (RNAi) technology has revolutionized functional genomic analyses in cell biology, including the study of cell division. In particular, the introduction of short interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) has facilitated loss-of-function studies in mammalian cell lines. We have pioneered the rapid and cost-efficient generation of libraries of endoribonuclease-prepared short interfering RNAs (esiRNAs) for large-scale genetic screens in mammalian tissue culture cells. Our first pilot screen of 5305 genes in human HeLa cells identified novel genes required for cell division, establishing esiRNA as a sophisticated method for loss-of-function screens. Future genome-wide studies with esiRNA, using image-based assays and video microscopy, will help provide novel insights on the molecular mechanisms that govern cell division in mammalian cells.

Original languageEnglish (US)
Pages (from-to)561-564
Number of pages4
JournalCell Cycle
Volume4
Issue number4
StatePublished - Apr 2005

Keywords

  • Gene silencing
  • Mitosis
  • Off-target effects
  • RNai
  • Screening
  • esiRNA
  • shRNA
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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