Functional high-and low affinity agonist binding sites at native dorsal horn AMPA receptors

V. Rybalchenko, R. Schlichter

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transmission and processing of nociceptive information in the superficial dorsal horn (DH) of the spinal cord involves activation of AMPA- type glutamate receptors (AMPARs). We have studied the properties of native AMPARs in freshly dissociated laminae I-II neurones from postnatal rats using a modified form of the concentration-clamp technique for fast agonist application. Analysis of kainic acid dose-response curves showed the existence of two types of functional agonist binding sites governing AMPAR activation. These sites differ by their affinity for the agonist. Depending on the neurotransmitter concentration reached in the synaptic cleft, the relative contribution of high affinity and low affinity sites might play an important role in the shaping of AMPAR-mediated postsynaptic currents.

Original languageEnglish (US)
Pages (from-to)1803-1806
Number of pages4
JournalNeuroReport
Volume10
Issue number8
StatePublished - Jun 3 1999

Fingerprint

AMPA Receptors
Glutamate Receptors
Binding Sites
Synaptic Potentials
Kainic Acid
Automatic Data Processing
Neurotransmitter Agents
Neurons
Spinal Cord Dorsal Horn

Keywords

  • Dorsal horn
  • Excitatory postsynaptic currents (EPSC)
  • Glutamate receptors
  • Kainic acid
  • Nociception
  • Pain
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Functional high-and low affinity agonist binding sites at native dorsal horn AMPA receptors. / Rybalchenko, V.; Schlichter, R.

In: NeuroReport, Vol. 10, No. 8, 03.06.1999, p. 1803-1806.

Research output: Contribution to journalArticle

@article{9e96bdd02ac04208b813065f169a52c7,
title = "Functional high-and low affinity agonist binding sites at native dorsal horn AMPA receptors",
abstract = "Transmission and processing of nociceptive information in the superficial dorsal horn (DH) of the spinal cord involves activation of AMPA- type glutamate receptors (AMPARs). We have studied the properties of native AMPARs in freshly dissociated laminae I-II neurones from postnatal rats using a modified form of the concentration-clamp technique for fast agonist application. Analysis of kainic acid dose-response curves showed the existence of two types of functional agonist binding sites governing AMPAR activation. These sites differ by their affinity for the agonist. Depending on the neurotransmitter concentration reached in the synaptic cleft, the relative contribution of high affinity and low affinity sites might play an important role in the shaping of AMPAR-mediated postsynaptic currents.",
keywords = "Dorsal horn, Excitatory postsynaptic currents (EPSC), Glutamate receptors, Kainic acid, Nociception, Pain, Spinal cord",
author = "V. Rybalchenko and R. Schlichter",
year = "1999",
month = "6",
day = "3",
language = "English (US)",
volume = "10",
pages = "1803--1806",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "8",

}

TY - JOUR

T1 - Functional high-and low affinity agonist binding sites at native dorsal horn AMPA receptors

AU - Rybalchenko, V.

AU - Schlichter, R.

PY - 1999/6/3

Y1 - 1999/6/3

N2 - Transmission and processing of nociceptive information in the superficial dorsal horn (DH) of the spinal cord involves activation of AMPA- type glutamate receptors (AMPARs). We have studied the properties of native AMPARs in freshly dissociated laminae I-II neurones from postnatal rats using a modified form of the concentration-clamp technique for fast agonist application. Analysis of kainic acid dose-response curves showed the existence of two types of functional agonist binding sites governing AMPAR activation. These sites differ by their affinity for the agonist. Depending on the neurotransmitter concentration reached in the synaptic cleft, the relative contribution of high affinity and low affinity sites might play an important role in the shaping of AMPAR-mediated postsynaptic currents.

AB - Transmission and processing of nociceptive information in the superficial dorsal horn (DH) of the spinal cord involves activation of AMPA- type glutamate receptors (AMPARs). We have studied the properties of native AMPARs in freshly dissociated laminae I-II neurones from postnatal rats using a modified form of the concentration-clamp technique for fast agonist application. Analysis of kainic acid dose-response curves showed the existence of two types of functional agonist binding sites governing AMPAR activation. These sites differ by their affinity for the agonist. Depending on the neurotransmitter concentration reached in the synaptic cleft, the relative contribution of high affinity and low affinity sites might play an important role in the shaping of AMPAR-mediated postsynaptic currents.

KW - Dorsal horn

KW - Excitatory postsynaptic currents (EPSC)

KW - Glutamate receptors

KW - Kainic acid

KW - Nociception

KW - Pain

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0033519566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033519566&partnerID=8YFLogxK

M3 - Article

C2 - 10501579

AN - SCOPUS:0033519566

VL - 10

SP - 1803

EP - 1806

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 8

ER -