Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo

S. Kaushal, G. E. Amiel, K. J. Guleserian, O. M. Shapira, T. Perry, F. W. Sutherland, E. Rabkin, A. M. Moran, F. J. Schoen, A. Atala, S. Soker, J. Bischoff, Jr Mayer J.E.

Research output: Contribution to journalArticle

673 Citations (Scopus)

Abstract

Arterial conduits are increasingly preferred for surgical bypass because of inherent functional properties conferred by arterial endothelial cells, especially nitric oxide production in response to physiologic stimuli. Here we tested whether endothelial progenitor cells (EPCs) can replace arterial endothelial cells and promote patency in tissue-engineered small-diameter blood vessels (4 mm). We isolated EPCs from peripheral blood of sheep, expanded them ex vivo and then seeded them on decellularized porcine iliac vessels. EPC-seeded grafts remained patent for 130 days as a carotid interposition graft in sheep, whereas non-seeded grafts occluded within 15 days. The EPC-explanted grafts exhibited contractile activity and nitric-oxide-mediated vascular relaxation that were similar to native carotid arteries. These results indicate that EPCs can function similarly to arterial endothelial cells and thereby confer longer vascular-graft survival. Due to their unique properties, EPCs might have other general applications for tissue-engineered structures and in treating vascular diseases.

Original languageEnglish (US)
Pages (from-to)1035-1040
Number of pages6
JournalNature Medicine
Volume7
Issue number9
DOIs
StatePublished - 2001

Fingerprint

Endothelial cells
Grafts
Transplants
Blood Vessels
Endothelial Cells
Sheep
Nitric Oxide
Graft Survival
Vascular Diseases
Carotid Arteries
Tissue
Endothelial Progenitor Cells
Swine
Blood vessels
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kaushal, S., Amiel, G. E., Guleserian, K. J., Shapira, O. M., Perry, T., Sutherland, F. W., ... Mayer J.E., J. (2001). Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo. Nature Medicine, 7(9), 1035-1040. https://doi.org/10.1038/nm0901-1035

Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo. / Kaushal, S.; Amiel, G. E.; Guleserian, K. J.; Shapira, O. M.; Perry, T.; Sutherland, F. W.; Rabkin, E.; Moran, A. M.; Schoen, F. J.; Atala, A.; Soker, S.; Bischoff, J.; Mayer J.E., Jr.

In: Nature Medicine, Vol. 7, No. 9, 2001, p. 1035-1040.

Research output: Contribution to journalArticle

Kaushal, S, Amiel, GE, Guleserian, KJ, Shapira, OM, Perry, T, Sutherland, FW, Rabkin, E, Moran, AM, Schoen, FJ, Atala, A, Soker, S, Bischoff, J & Mayer J.E., J 2001, 'Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo', Nature Medicine, vol. 7, no. 9, pp. 1035-1040. https://doi.org/10.1038/nm0901-1035
Kaushal, S. ; Amiel, G. E. ; Guleserian, K. J. ; Shapira, O. M. ; Perry, T. ; Sutherland, F. W. ; Rabkin, E. ; Moran, A. M. ; Schoen, F. J. ; Atala, A. ; Soker, S. ; Bischoff, J. ; Mayer J.E., Jr. / Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo. In: Nature Medicine. 2001 ; Vol. 7, No. 9. pp. 1035-1040.
@article{823cace898974cd0878d497a10ec4713,
title = "Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo",
abstract = "Arterial conduits are increasingly preferred for surgical bypass because of inherent functional properties conferred by arterial endothelial cells, especially nitric oxide production in response to physiologic stimuli. Here we tested whether endothelial progenitor cells (EPCs) can replace arterial endothelial cells and promote patency in tissue-engineered small-diameter blood vessels (4 mm). We isolated EPCs from peripheral blood of sheep, expanded them ex vivo and then seeded them on decellularized porcine iliac vessels. EPC-seeded grafts remained patent for 130 days as a carotid interposition graft in sheep, whereas non-seeded grafts occluded within 15 days. The EPC-explanted grafts exhibited contractile activity and nitric-oxide-mediated vascular relaxation that were similar to native carotid arteries. These results indicate that EPCs can function similarly to arterial endothelial cells and thereby confer longer vascular-graft survival. Due to their unique properties, EPCs might have other general applications for tissue-engineered structures and in treating vascular diseases.",
author = "S. Kaushal and Amiel, {G. E.} and Guleserian, {K. J.} and Shapira, {O. M.} and T. Perry and Sutherland, {F. W.} and E. Rabkin and Moran, {A. M.} and Schoen, {F. J.} and A. Atala and S. Soker and J. Bischoff and {Mayer J.E.}, Jr",
year = "2001",
doi = "10.1038/nm0901-1035",
language = "English (US)",
volume = "7",
pages = "1035--1040",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo

AU - Kaushal, S.

AU - Amiel, G. E.

AU - Guleserian, K. J.

AU - Shapira, O. M.

AU - Perry, T.

AU - Sutherland, F. W.

AU - Rabkin, E.

AU - Moran, A. M.

AU - Schoen, F. J.

AU - Atala, A.

AU - Soker, S.

AU - Bischoff, J.

AU - Mayer J.E., Jr

PY - 2001

Y1 - 2001

N2 - Arterial conduits are increasingly preferred for surgical bypass because of inherent functional properties conferred by arterial endothelial cells, especially nitric oxide production in response to physiologic stimuli. Here we tested whether endothelial progenitor cells (EPCs) can replace arterial endothelial cells and promote patency in tissue-engineered small-diameter blood vessels (4 mm). We isolated EPCs from peripheral blood of sheep, expanded them ex vivo and then seeded them on decellularized porcine iliac vessels. EPC-seeded grafts remained patent for 130 days as a carotid interposition graft in sheep, whereas non-seeded grafts occluded within 15 days. The EPC-explanted grafts exhibited contractile activity and nitric-oxide-mediated vascular relaxation that were similar to native carotid arteries. These results indicate that EPCs can function similarly to arterial endothelial cells and thereby confer longer vascular-graft survival. Due to their unique properties, EPCs might have other general applications for tissue-engineered structures and in treating vascular diseases.

AB - Arterial conduits are increasingly preferred for surgical bypass because of inherent functional properties conferred by arterial endothelial cells, especially nitric oxide production in response to physiologic stimuli. Here we tested whether endothelial progenitor cells (EPCs) can replace arterial endothelial cells and promote patency in tissue-engineered small-diameter blood vessels (4 mm). We isolated EPCs from peripheral blood of sheep, expanded them ex vivo and then seeded them on decellularized porcine iliac vessels. EPC-seeded grafts remained patent for 130 days as a carotid interposition graft in sheep, whereas non-seeded grafts occluded within 15 days. The EPC-explanted grafts exhibited contractile activity and nitric-oxide-mediated vascular relaxation that were similar to native carotid arteries. These results indicate that EPCs can function similarly to arterial endothelial cells and thereby confer longer vascular-graft survival. Due to their unique properties, EPCs might have other general applications for tissue-engineered structures and in treating vascular diseases.

UR - http://www.scopus.com/inward/record.url?scp=0034785776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034785776&partnerID=8YFLogxK

U2 - 10.1038/nm0901-1035

DO - 10.1038/nm0901-1035

M3 - Article

VL - 7

SP - 1035

EP - 1040

JO - Nature Medicine

JF - Nature Medicine

SN - 1078-8956

IS - 9

ER -