A furosemide-sensitive, ouabain-insensitive [86Rb+] uptake is described in glioma cells in culture which is dependent upon external Na+, K+, and Cl- concentrations. This transport activity was also inhibited by bumetanide at 100-fold lower concentrations than furosemide. Furosemide-sensitive swelling of glioma cells is demonstrated and this activity is dependent upon external Na+ and K+ in a manner similar to [86Rb+] uptake. This transport activity was not detected in neuroblastoma cells and the possible relevance of these findings to extracellular K+ buffering by glia is discussed.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Nov 16 1982|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology