Gα(q) and Gα13 regulate NHE-1 and intracellular calcium in epithelial cells

K. Kitamura, W. D. Singer, A. Cano, R. T. Miller

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Abstract

To understand the mechanisms by which G protein-coupled signaling systems regulate NHE-1 in epithelial cells, we expressed Gα(q) and Gα13 in a renal epithelial cell line. We studied two signaling systems that have been implicated in NHE-1 regulation [intracellular Ca (Ca(i)) and phospholipase C activity] and measured NHE-1 activity, mRNA, and antigen. Expression of α(qWT) and α(qQ209L) (a GTPase-deficient mutant) increased basal Ca(i) and altered the kinetics of the bradykinin-induced Ca(i) signal. The initial bradykinin-induced spike in Ca(i) was prolonged and the plateau was higher in cells expressing α(qWT) and α(qQ209L) than in control cells. Cells expressing α(13WT) also had a higher basal Ca(i) and plateau after stimulation by bradykinin, but Ca release from intracellular stores was similar to that in control cells. Expression of all three α-chains increased NHE-1 activity, antigen, and mRNA. The αq(Q209L) had the greatest effect increasing activity by approximately twofold. The α(13WT) increased NHE-1 activity by ~1.5-fold, and α(qWT) increased activity 1.2-fold. These studies demonstrate that α(q) and α13 alter regulation of Ca(i) but by different mechanisms. The Ca signal or another signal generated by α(q) and α13 regulate(s) NHE-1 at the levels of activity, antigen, and mRNA.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume268
Issue number1 37-1
StatePublished - 1995

Fingerprint

Bradykinin
bradykinin
epithelial cells
Epithelial Cells
Calcium
calcium
Antigens
Messenger RNA
antigens
Cells
plateaus
GTP Phosphohydrolases
Type C Phospholipases
cells
GTP-Binding Proteins
phospholipase C
guanosinetriphosphatase
kidney cells
G-proteins
Kinetics

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Gα(q) and Gα13 regulate NHE-1 and intracellular calcium in epithelial cells. / Kitamura, K.; Singer, W. D.; Cano, A.; Miller, R. T.

In: American Journal of Physiology - Cell Physiology, Vol. 268, No. 1 37-1, 1995.

Research output: Contribution to journalArticle

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