G Protein β Subunit Types Differentially Interact with a Muscarinic Receptor but Not Adenylyl Cyclase Type II or Phospholipase C-β2/3

Yongmin Hou, Vanessa Chang, Austin B. Capper, Ronald Taussig, N. Gautam

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In comparison with the α subunit of G proteins, the role of the β subunit in signaling is less well understood. During the regulation of effectors by the βγ complex, it is known that the β subunit contacts effectors directly, whereas the role of the β subunit is undefined in receptor-G protein interaction. Among the five G protein β subunits known, the β4 subunit type is the least studied. We compared the ability of βγ complexes containing β4 and the well characterized β1 to stimulate three different effectors: phospholipase C-β2, phospholipase C-β3, and adenylyl cyclase type II. β 4γ2 and β1γ2 activated all three of these effectors with equal efficacy. However, nucleotide exchange in a G protein constituting αoβ 4γ2 was stimulated significantly more by the M2 muscarinic receptor compared with αoβ1γ 2. Because αo forms heterotrimers with β 4γ2 and β1γ2 equally well, these results show that the β subunit type plays a direct role in the receptor activation of a G protein.

Original languageEnglish (US)
Pages (from-to)19982-19988
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number23
DOIs
StatePublished - Jun 8 2001

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Protein Subunits
Type C Phospholipases
Muscarinic Receptors
GTP-Binding Proteins
Adenylyl Cyclases
Gi-Go GTP-Binding Protein alpha Subunits
Muscarinic M2 Receptors
Nucleotides
Chemical activation

ASJC Scopus subject areas

  • Biochemistry

Cite this

G Protein β Subunit Types Differentially Interact with a Muscarinic Receptor but Not Adenylyl Cyclase Type II or Phospholipase C-β2/3. / Hou, Yongmin; Chang, Vanessa; Capper, Austin B.; Taussig, Ronald; Gautam, N.

In: Journal of Biological Chemistry, Vol. 276, No. 23, 08.06.2001, p. 19982-19988.

Research output: Contribution to journalArticle

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