G protein-coupled receptor 120 signaling regulates ghrelin secretion in vivo and in vitro

Zhi Gong, Makoto Yoshimura, Sayaka Aizawa, Reiko Kurotani, Jeffrey M. Zigman, Takafumi Sakai, Ichiro Sakata

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is produced predominantly in the stomach. It has been reported that endogenous ghrelin levels are increased by fasting and decreased immediately after feeding and that fasting-induced ghrelin release is controlled by the sympathetic nervous system. However, the mechanisms of plasma ghrelin decrement after feeding are poorly understood. Here, we studied the control of ghrelin secretion using ghrelin-producing cell lines and found that these cells express high levels of mRNA encoding G-protein coupled receptor 120 (GPR120). Addition of GW-9508 (a GPR120 chemical agonist) and α-linolenic acid (a natural ligand for GPR120) inhibited the secretion of ghrelin by ~50 and 70%, respectively. However, the expression levels of preproghrelin and ghrelin O-acyltransferase (GOAT) mRNAs were not influenced by GW-9508. In contrast, the expression levels of prohormone convertase 1 were decreased significantly by GW-9508 incubation. Moreover, we observed that the inhibitory effect of GW-9508 on ghrelin secretion was blocked by a small interfering RNA (siRNA) targeting the sequence of GPR120. Furthermore, pretreatment with GW-9508 blocked the effect of the norepinephrine (NE)-induced ghrelin elevation in ghrelin cell lines. In addition, we showed that GW-9508 inhibited ghrelin secretion via extracellular signal-regulated kinase activity in ghrelin cell lines. Finally, we found that GW-9508 decreased plasma ghrelin levels in mice. These results suggest that the decrease of ghrelin secretion after feeding is induced partially by long-chain fatty acids that act directly on gastric GPR120-expressing ghrelin cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume306
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

Ghrelin
G-Protein-Coupled Receptors
In Vitro Techniques
Cell Line
Fasting
Stomach
Proprotein Convertase 1
Ghrelin Receptor
Ligands
Acyltransferases
Messenger RNA
alpha-Linolenic Acid
Sympathetic Nervous System
Extracellular Signal-Regulated MAP Kinases

Keywords

  • G protein-coupled receptor 120
  • Ghrelin
  • Ghrelin cell line
  • Secretion

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

G protein-coupled receptor 120 signaling regulates ghrelin secretion in vivo and in vitro. / Gong, Zhi; Yoshimura, Makoto; Aizawa, Sayaka; Kurotani, Reiko; Zigman, Jeffrey M.; Sakai, Takafumi; Sakata, Ichiro.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 306, No. 1, 01.01.2014.

Research output: Contribution to journalArticle

Gong, Zhi ; Yoshimura, Makoto ; Aizawa, Sayaka ; Kurotani, Reiko ; Zigman, Jeffrey M. ; Sakai, Takafumi ; Sakata, Ichiro. / G protein-coupled receptor 120 signaling regulates ghrelin secretion in vivo and in vitro. In: American Journal of Physiology - Endocrinology and Metabolism. 2014 ; Vol. 306, No. 1.
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