G protein-coupled receptor genes in the FANTOM2 database

Yuka Kawasawa, Louise M. McKenzie, David P. Hill, Hidemasa Bono, Takahiro Arakawa, Piero Carninci, Jun Kawai, Yoshihide Hayashizaki, Masashi Yanagisawa

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

G protein-coupled receptors (GPCRs) comprise the largest family of receptor proteins in mammals and play important roles in many physiological and pathological processes. Gene expression of GPCRs is temporally and spatially regulated, and many splicing variants are also described. In many instances, different expression profiles of GPCR gene are accountable for the changes of its biological function. Therefore, it is intriguing to assess the complexity of the transcriptome of GPCRs in various mammalian organs. In this study, we took advantage of the FANTOM2 (Functional Annotation Meeting of Mouse cDNA 2) project, which aimed to collect full-length cDNAs inclusively from mouse tissues, and found 410 candidate GPCR cDNAs. Clustering of these clones into transcriptional units (TUs) reduced this number to 213. Out of these, 165 genes were represented within the known 308 GPCRs in the Mouse Genome Informatics (MGI) resource. The remaining 48 genes were new to mouse, and 14 of them had no clear mammalian ortholog. To dissect the detailed characteristics of each transcript, tissue distribution pattern and alternative splicing were also ascertained. We found many splicing variants of GPCRs that may have a relevance to disease occurrence. In addition, the difficulty in cloning tissue-specific and infrequently transcribed GPCRs is discussed further.

Original languageEnglish (US)
Pages (from-to)1466-1477
Number of pages12
JournalGenome Research
Volume13
Issue number6 B
DOIs
StatePublished - Jun 1 2003

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Kawasawa, Y., McKenzie, L. M., Hill, D. P., Bono, H., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., & Yanagisawa, M. (2003). G protein-coupled receptor genes in the FANTOM2 database. Genome Research, 13(6 B), 1466-1477. https://doi.org/10.1101/gr.1087603