G proteins and regulation of adenylyl cyclases

Alfred G. Gilman

Research output: Contribution to journalArticle

Abstract

Members of two classes of G proteins, Gs and Gi regulate adenylyl cyclases directly. The activated α subunit of Gs stimulates all mammalian adenylyl cyclases, while the α subunits of the three Gi proteins inhibit certain isoforms. Other adenylyl cyclases are inhibited by G protein βγ subunits or are activated conditionally by βγ in the presence of G. We are taking structural and mutagenic approaches to understand these regulatory interactions. High resolution crystal structures of Giα1 in its GTPγS, GDP, and GDP + AlF4- bound states have been solved, as has the structure of the Giα1β1γ2 heterotrimer. The conformational changes that drive the sequential protein-protein interactions that characterize the system and that permit slow hydrolysis of GTP have been visualized. The G protein β subunit contains 7 so-called WD repeats and its structure is that of a 7-fold β propeller. Biochemical and structural approaches to adenylyl cyclases are limited by the low abundance and hydrophobicity of these enzymes. We have synthesized and purified a 55-kDa soluble form of mammalian adenylyl cyclase devoid of all of its hydrophobic domains. This protein retains the regulatory features that are shared by mammalian adenylyl cyclases. The two cytosolic domains of the enzyme can also be synthesized separately, purified, and reconstituted to yield G- and forskolin-stimulated enzymatic activity. This system will greatly facilitate biochemical, genetic, and structural approaches to understanding mechanisms of regulation.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

adenylate cyclase
G-proteins
GTP-Binding Proteins
Adenylyl Cyclases
Protein Subunits
protein subunits
Proteins
forskolin
regulatory proteins
protein-protein interactions
Colforsin
Propellers
Enzymes
Hydrophobicity
enzymes
crystal structure
Guanosine Triphosphate
hydrophobicity
Hydrophobic and Hydrophilic Interactions
molecular genetics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

G proteins and regulation of adenylyl cyclases. / Gilman, Alfred G.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Gilman, Alfred G. / G proteins and regulation of adenylyl cyclases. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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