GABA inhibition of lateral hypothalamic neurons: Role of reticular thalamic afferents

Frank C. Barone, Cheng Jung-Tung, Matthew J. Wayner

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

GABA and reticular thalamic (RT) stimulation induced inhibition of lateral hypothalamic (LH) neuronal activity was studied to determine if RT inhibitory input to the LH is mediated by stimulation of GABA receptors. Seven barrel electrodes were utilized to record simultaneously from the LH during microiontophoretic application of GABA, glycine, bicuculline, picrotoxin, and electrical stimulation of the RT. GABA produced an ejection current-related decrease in LH neuronal activity that was antagonized, in a dose-related manner, by the simultaneous administration of picrotoxin or bicuculline. LH neurons were much less sensitive to glycine inhibition which also was relatively insensitive to the GABA receptor antagonists. RT electrical stimulation provided a short latency inhibitory input to LH neurons that was mimicked by the microiontophoretic administration of GABA. In addition, the microiontophoretic application of picrotoxin or bicuculline blocked the RT stimulation induced inhibition of LH neuronal activity. These data indicate that the inhibitory effects of both GABA and RT synaptic inputs onto LH neurons are mediated by stimulation of GABAA receptors and substantiates the involvement of GABA as the inhibitory transmitter of thalamic to hypothalamic projections.

Original languageEnglish (US)
Pages (from-to)699-708
Number of pages10
JournalBrain Research Bulletin
Volume33
Issue number6
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

gamma-Aminobutyric Acid
Neurons
Picrotoxin
Bicuculline
Glycine
Electric Stimulation
GABA Antagonists
GABA Receptors
GABA-A Receptors
Electrodes

Keywords

  • Bicuculline
  • GABA
  • Lateral hypothalamus
  • Picrotoxin
  • Reticular thalamic nucleus
  • Thalamus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

GABA inhibition of lateral hypothalamic neurons : Role of reticular thalamic afferents. / Barone, Frank C.; Jung-Tung, Cheng; Wayner, Matthew J.

In: Brain Research Bulletin, Vol. 33, No. 6, 1994, p. 699-708.

Research output: Contribution to journalArticle

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