Galactosyltransferase activities in human sera: Detection of a cancer-associated isoenzyme

Daniel K. Podolsky, Milton M. Weiser

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Sera from normal subjects and cancer patients were evaluated for galactosyltransferase activity including kinetic and electrophoretic properties. Discontinuous polyacrylamide electrophoresis of whole sera demonstrated the presence of an additional peak of enzyme activity in cancer sera. The majority of recovered enzymatic activity in both normal and cancer sera ran as a broad band of activity behind an albumin standard; the cancerassociated peak was slower moving. Techniques have been developed for the rapid assay of galactosyltransferase activity from gel slices which permit detection of both peaks of activity.

Original languageEnglish (US)
Pages (from-to)545-551
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume65
Issue number2
DOIs
StatePublished - Jul 22 1975

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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