Galectin-3 blockade inhibits cardiac inflammation and fibrosis in experimental hyperaldosteronism and hypertension

Ernesto Martínez-Martínez, Laurent Calvier, Amaya Fernández-Celis, Elodie Rousseau, Raquel Jurado-López, Luciana V. Rossoni, Frederic Jaisser, Faiez Zannad, Patrick Rossignol, Victoria Cachofeiro, Natalia López-Andrés

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

Hypertensive cardiac remodeling is accompanied by molecular inflammation and fibrosis, 2 mechanisms that finally affect cardiac function. At cardiac level, aldosterone promotes inflammation and fibrosis, although the precise mechanisms are still unclear. Galectin-3 (Gal-3), a β-galactoside-binding lectin, is associated with inflammation and fibrosis in the cardiovascular system. We herein investigated whether Gal-3 inhibition could block aldosterone-induced cardiac inflammation and fibrosis and its potential role in cardiac damage associated with hypertension. Aldosterone-salt-treated rats presented hypertension, cardiac inflammation, and fibrosis that were prevented by the pharmacological inhibition of Gal-3 with modified citrus pectin. Cardiac inflammation and fibrosis presented in spontaneously hypertensive rats were prevented by modified citrus pectin treatment, whereas Gal-3 blockade did not modify blood pressure levels. In the absence of blood pressure modifications, Gal-3 knockout mice were resistant to aldosterone-induced cardiac inflammation. In human cardiac fibroblasts, aldosterone increased Gal-3 expression via its mineralocorticoid receptor. Gal-3 and aldosterone enhanced proinflammatory and profibrotic markers, as well as metalloproteinase activities in human cardiac fibroblasts, effects that were not observed in Gal-3-silenced cells treated with aldosterone. In experimental hyperaldosteronism, the increase in Gal-3 expression was associated with cardiac inflammation and fibrosis, alterations that were prevented by Gal-3 blockade independently of blood pressure levels. These data suggest that Gal-3 could be a new molecular mechanism linking cardiac inflammation and fibrosis in situations with high-aldosterone levels, such as hypertension.

Original languageEnglish (US)
Pages (from-to)767-775
Number of pages9
JournalHypertension
Volume66
Issue number4
DOIs
StatePublished - Oct 11 2015
Externally publishedYes

Keywords

  • aldosterone
  • fibrosis
  • galectin 3
  • hypertension
  • inflammation

ASJC Scopus subject areas

  • Internal Medicine

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