Gankyrin facilitates follicle-stimulating hormone-driven ovarian cancer cell proliferation through the PI3K/AKT/HIF-1α/cyclin D1 pathway

J. Chen, M. Bai, C. Ning, B. Xie, J. Zhang, H. Liao, J. Xiong, X. Tao, D. Yan, X. Xi, X. Chen, Y. Yu, R. C. Bast, Z. Zhang, Y. Feng, W. Zheng

Research output: Contribution to journalArticle

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Abstract

Gankyrin is a regulatory subunit of the 26kD proteasome complex. As a novel oncoprotein, gankyrin is expressed aberrantly in cancers from several different sites and has been shown to contribute to oncogenesis in endometrial and cervical carcinomas. Neither gankyrin's contribution to the development of epithelial ovarian cancer nor its interaction with follicle-stimulating hormone (FSH)-driven proliferation in ovarian cancer has been studied. Here we have found that gankyrin is overexpressed in ovarian cancers compared with benign ovarian cystadenomas and that gankyrin regulates FSH upregulation of cyclin D1. Importantly, gankyrin regulates PI3K/AKT signaling by downregulating PTEN. Prolonged AKT activation by FSH stimulation of the FSH receptor (FSHR) promotes gankyrin expression, which, in turn, enhances AKT activation by inhibiting PTEN. Overexpression of gankyrin decreases hypoxia inducible factor-1α (HIF-1α) protein levels, but has little effect on HIF-1α mRNA levels, which could be attributed to gankyrin mediating HIF-1α protein stability via the ubiquitin-proteasome pathway. Reduction in HIF-1α protein stability led to attenuation of the binding with cyclin D1 promoter, resulted in abolishment of the negative regulation of cyclin D1 by HIF-1α, which promotes proliferation of ovarian cancer cells. Our results document that gankyrin regulates HIF-1α protein stability and cyclin D1 expression, ultimately mediating FSH-driven ovarian cancer cell proliferation.

Original languageEnglish (US)
Pages (from-to)2506-2517
Number of pages12
JournalOncogene
Volume35
Issue number19
DOIs
StatePublished - May 12 2016

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Hypoxia-Inducible Factor 1
Cyclin D1
Follicle Stimulating Hormone
Phosphatidylinositol 3-Kinases
Ovarian Neoplasms
Cell Proliferation
Protein Stability
Proteasome Endopeptidase Complex
FSH Receptors
Cystadenoma
Oncogene Proteins
Endometrial Neoplasms
Ubiquitin
Carcinogenesis
Up-Regulation
Down-Regulation
Messenger RNA
Neoplasms
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Gankyrin facilitates follicle-stimulating hormone-driven ovarian cancer cell proliferation through the PI3K/AKT/HIF-1α/cyclin D1 pathway. / Chen, J.; Bai, M.; Ning, C.; Xie, B.; Zhang, J.; Liao, H.; Xiong, J.; Tao, X.; Yan, D.; Xi, X.; Chen, X.; Yu, Y.; Bast, R. C.; Zhang, Z.; Feng, Y.; Zheng, W.

In: Oncogene, Vol. 35, No. 19, 12.05.2016, p. 2506-2517.

Research output: Contribution to journalArticle

Chen, J, Bai, M, Ning, C, Xie, B, Zhang, J, Liao, H, Xiong, J, Tao, X, Yan, D, Xi, X, Chen, X, Yu, Y, Bast, RC, Zhang, Z, Feng, Y & Zheng, W 2016, 'Gankyrin facilitates follicle-stimulating hormone-driven ovarian cancer cell proliferation through the PI3K/AKT/HIF-1α/cyclin D1 pathway', Oncogene, vol. 35, no. 19, pp. 2506-2517. https://doi.org/10.1038/onc.2015.316
Chen, J. ; Bai, M. ; Ning, C. ; Xie, B. ; Zhang, J. ; Liao, H. ; Xiong, J. ; Tao, X. ; Yan, D. ; Xi, X. ; Chen, X. ; Yu, Y. ; Bast, R. C. ; Zhang, Z. ; Feng, Y. ; Zheng, W. / Gankyrin facilitates follicle-stimulating hormone-driven ovarian cancer cell proliferation through the PI3K/AKT/HIF-1α/cyclin D1 pathway. In: Oncogene. 2016 ; Vol. 35, No. 19. pp. 2506-2517.
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AU - Xie, B.

AU - Zhang, J.

AU - Liao, H.

AU - Xiong, J.

AU - Tao, X.

AU - Yan, D.

AU - Xi, X.

AU - Chen, X.

AU - Yu, Y.

AU - Bast, R. C.

AU - Zhang, Z.

AU - Feng, Y.

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