Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini

Peggy Loessberg Stauffer, Hong Zhao, Katherine Luby-Phelps, Robert L. Moss, Robert A. Star, Shmuel Muallem

Research output: Contribution to journalArticle

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Abstract

Global (all cells in an acinus) and focal (1-2 out of 10-15 cells) stimulation of pancreatic acini with bombesin or t-butyloxycarbonyl-Tyr(SO3)-Nle-Gly-Tyr-Asp-2-phenylethyl ester (CCKJ) together with modulation of gap junction (GJ) permeability by octanol and NO2 - was used to study the role of GJ permeability in controlling [Ca2+]i oscillations and enzyme secretion. GJ permeability was quantitated by measuring fluorescence recovery after photobleaching. Octanol at 0.5 mM markedly reduced, whereas 15 mM NO2 - increased GJ permeability. Focal application of bombesin caused synchronized oscillations in the entire acinus, whereas global stimulation resulted in asynchronous oscillations. Increasing GJ permeability with NO2 - had no effect on bombesin-evoked [Ca2+]i- oscillations. Octanol inhibited ongoing oscillations evoked by focal or global bombesin stimulation. However, when GJ were blocked prior to stimulation, subsequent global stimulation with bombesin induced long-lasting oscillations in all cells. Re-establishing GJ communication for as little as 37.5 s conferred GJ dependence on the order and time of [Ca2+]i spiking evoked by global bombesin stimulation. Focal and global stimulation with CCKJ gave different patterns of [Ca2+]i- oscillations. However, in contrast to bombesin, inhibition of GJ with octanol had no effect on oscillations induced by global CCKJ stimulation. Increasing GJ permeability with NO2 - synchronized CCKJ-stimulated oscillations by equalizing the amplitude and increasing the frequency in all cells within an acinus. These observations suggest that amplitude and frequency of [Ca2+]i oscillations can be regulated independently of each other, and that GJ permeable molecules modulate the frequency of [Ca2+]i-oscillation in an agonist-specific manner. Regardless of the agonist, increasing the frequency of oscillations by modulation of GJ permeability correlated with an increased enzyme secretion.

Original languageEnglish (US)
Pages (from-to)19769-19775
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number26
StatePublished - Sep 15 1993

Fingerprint

Bombesin
Gap Junctions
Octanols
Communication
Enzymes
Permeability
Modulation
Acinar Cells
Photobleaching
Esters
Fluorescence Recovery After Photobleaching
Fluorescence
Recovery
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Stauffer, P. L., Zhao, H., Luby-Phelps, K., Moss, R. L., Star, R. A., & Muallem, S. (1993). Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini. Journal of Biological Chemistry, 268(26), 19769-19775.

Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini. / Stauffer, Peggy Loessberg; Zhao, Hong; Luby-Phelps, Katherine; Moss, Robert L.; Star, Robert A.; Muallem, Shmuel.

In: Journal of Biological Chemistry, Vol. 268, No. 26, 15.09.1993, p. 19769-19775.

Research output: Contribution to journalArticle

Stauffer, PL, Zhao, H, Luby-Phelps, K, Moss, RL, Star, RA & Muallem, S 1993, 'Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini', Journal of Biological Chemistry, vol. 268, no. 26, pp. 19769-19775.
Stauffer, Peggy Loessberg ; Zhao, Hong ; Luby-Phelps, Katherine ; Moss, Robert L. ; Star, Robert A. ; Muallem, Shmuel. / Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 26. pp. 19769-19775.
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AU - Muallem, Shmuel

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